*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , RD , HeLa(50 μM chloroquine treatment for 24 hr)
Rat, Zebrafish, Bovine, Rhesus Monkey(>80% identity)
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human LC3A. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
microtubule associated protein 1 light chain 3 alpha,ATG8E,LC3,LC3A,MAP1ALC3,MAP1BLC3
Cytoplasm , Endomembrane system; Lipid-anchor , Cytoplasmic vesicle , autophagosome membrane; Lipid-anchor
MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
GTX132889 WB Image
Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3A antibody (GTX132889) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX132889 WB Image
RD and untreated (–) or treated (+) HeLa whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3A antibody (GTX132889) diluted at 1:1000.