GeneTex
United States (US)

LC3A antibody

GTX31898_1549_IHC-P_20180221_18121410_745.jpg
GTX31898_1550_IHC-P_20180221_18121410_326.jpg
GTX31898_3044_WB_20180221_18121410_464.jpg

Cat No. GTX31898

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , HEK293 Cell Lysate(GTX27902) , HeLa Cell Lysate

Specificity/Sensitivity

LC3A antibody is human specific. At least two isoforms of LC3A are known to exist. LC3A antibody is predicted to not cross-react with LC3B or LC3C.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

LC3A antibody was raised against a 10 amino acid peptide near the amino terminus of human LC3A.The immunogen is located within the first 50 amino acids of LC3A.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Microtubule Associated Protein 1 Light Chain 3 Alpha,Atg8E,Lc3,Lc3A,Map1Alc3,Map1Blc3,Map1Lc3A

Background

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. [provided by RefSeq, Feb 2012]

Database

Research Area

DATA IMAGES
GTX31898_1549_IHC-P_20180221_18121410_745.jpg

GTX31898 IHC-P Image

IHC-P analysis of human brain tissue using GTX31898 LC3A antibody.
Working concentration : 5 μg/ml

GTX31898_1550_IHC-P_20180221_18121410_326.jpg

GTX31898 IHC-P Image

IHC-P analysis of human brain tissue using GTX31898 LC3A antibody.
Working concentration : 20 μg/ml

GTX31898_3044_WB_20180221_18121410_464.jpg

GTX31898 WB Image

WB analysis of 293 cell lysate using GTX31898 LC3A antibody.
Working concentration : 1 μg/ml

Package List Price ($)
$ 329