GeneTex
United States (US)

LC3A antibody

GTX31934_1614_IHC-P_20180221_18121410_339.jpg
GTX31934_3084_WB_20180221_18121410_231.jpg

Cat No. GTX31934

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 1 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

Human Brain Tissue Lysate(GTX27918)

Specificity/Sensitivity

Multiple isoforms are known to exist. This antibody is predicted to detect LC3A, LC3B, and LC3C.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

LC3A antibody was raised against a 12 amino acid peptide near the center of human LC3AA.The immunogen is located within amino acids 20 - 70 of LC3A.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Microtubule Associated Protein 1 Light Chain 3 Alpha,Atg8E,Lc3,Lc3A,Map1Alc3,Map1Blc3,Map1Lc3A

Background

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. [provided by RefSeq, Feb 2012]

Database

Research Area

DATA IMAGES
GTX31934_1614_IHC-P_20180221_18121410_339.jpg

GTX31934 IHC-P Image

IHC-P analysis of rat brain tissue using GTX31934 LC3A antibody.
Working concentration : 5 μg/ml

GTX31934_3084_WB_20180221_18121410_231.jpg

GTX31934 WB Image

WB analysis of human brain tissue lysate using GTX31934 LC3A antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Package List Price ($)
$ 329