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LC3A antibody

Orthogonal
Anti-LC3A antibody used in IHC (Paraffin sections) (IHC-P). GTX48634
Anti-LC3A antibody used in Western Blot (WB). GTX48634
Anti-LC3A antibody used in Western Blot (WB). GTX48634
Anti-LC3A antibody used in IHC (Paraffin sections) (IHC-P). GTX48634
Anti-LC3A antibody used in Western Blot (WB). GTX48634
Anti-LC3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48634

Cat. No. GTX48634

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat, Zebrafish, Bovine
Package
50 μl ($399)
See all LC3A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2500
ICC/IF 1:100
IHC-P 1:100 - 1:400
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Product Note

Although specificity between LC3A and LC3B has not been tested, this antibody was created to a peptide that has 100% identity to LC3A and 62% identity to LC3B.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Genomic sequence made to an N-terminal portion of the human LC3A protein [Swiss-Prot# Q9H492].

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10625700

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

microtubule associated protein 1 light chain 3 alpha , ATG8E , LC3 , LC3A , MAP1ALC3 , MAP1BLC3

Cellular Localization

Cytoplasm, cytoskeleton

Background

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LC3A antibody used in IHC (Paraffin sections) (IHC-P). GTX48634

GTX48634 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX48634 LC3A antibody.
Dilution : 1:300

Anti-LC3A antibody used in Western Blot (WB). GTX48634

GTX48634 WB Image

WB analysis of Neuro2a cell lysate using GTX48634 LC3A antibody.

Anti-LC3A antibody used in Western Blot (WB). GTX48634

GTX48634 WB Image

WB analysis of human brain tissue lysate using GTX48634 LC3A antibody.

Anti-LC3A antibody used in IHC (Paraffin sections) (IHC-P). GTX48634

GTX48634 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX48634 LC3A antibody.
Dilution : 1:300

Anti-LC3A antibody used in Western Blot (WB). GTX48634

GTX48634 WB Image

Orthogonal

WB analysis of HeLa and Neuro2a cells treated with or without 50 uM chloroquine(CQ) for 24 hours using GTX48634 LC3A antibody.
Dilution : 2 μg/ml

Anti-LC3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX48634

GTX48634 ICC/IF Image

ICC/IF analysis of HeLa cells treated with 50uM chloroquine using GTX48634 LC3A antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI

REFERENCE

Application Reference

Miao CC et al. Autophagy 2022; 18 (4) : 921-934 LC3A-mediated autophagy regulates lung cancer cell plasticity.
Application : WB
Reactivity : Human
Jin J et al. Ren Fail 2019; 41 (1) : 294-302 Autophagy activity and expression pattern of autophagy-related markers in the podocytes of patients with lupus nephritis: association with pathological classification.
Application : IHC-P
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399