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LC3B antibody [5H12]

Anti-LC3B antibody [5H12] used in Flow cytometry (FACS). GTX60594
Anti-LC3B antibody [5H12] used in Western Blot (WB). GTX60594
Anti-LC3B antibody [5H12] used in ELISA (ELISA). GTX60594
Anti-LC3B antibody [5H12] used in Western Blot (WB). GTX60594

Cat. No. GTX60594

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5H12

Isotype

 IgG1

Application

 WB, FACS, ELISA

Reactivity

 Human
Package
100 μl ($409)
See all LC3B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human MAP1LC3B expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

microtubule associated protein 1 light chain 3 beta , ATG8F , LC3B , MAP1A/1BLC3 , MAP1LC3B-a

Cellular Localization

Cytoplasm, cytoskeleton

Background

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-LC3B antibody [5H12] used in Flow cytometry (FACS). GTX60594

GTX60594 FACS Image

FACS analysis of HeLa cells using GTX60594 LC3B antibody [5H12].
Green : LC3B
Red : negative control

Anti-LC3B antibody [5H12] used in Western Blot (WB). GTX60594

GTX60594 WB Image

WB analysis of human LC3B (AA: 1-125) recombinant protein using GTX60594 LC3B antibody [5H12].

Anti-LC3B antibody [5H12] used in ELISA (ELISA). GTX60594

GTX60594 ELISA Image

ELISA analysis of antigen using GTX60594 LC3B antibody [5H12].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-LC3B antibody [5H12] used in Western Blot (WB). GTX60594

GTX60594 WB Image

WB analysis of HEK293 (1) and LC3B (AA: 1-125)-hIgGFc transfected HEK293 (2) cell lysate using GTX60594 LC3B antibody [5H12].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 409