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LC3B antibody [GT3612]

Orthogonal Overexpression
Anti-LC3B antibody [GT3612] used in Western Blot (WB). GTX632501
Anti-LC3B antibody [GT3612] used in Western Blot (WB). GTX632501
Anti-LC3B antibody [GT3612] used in Western Blot (WB). GTX632501
Anti-LC3B antibody [GT3612] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632501

Cat. No. GTX632501

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT3612

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all LC3B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

LC3B-transfected 293T , HepG2 (3 μM Thapsigargin treated for 12hr) , HeLa (50 μM chloroquine treated for 24hr)

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Pig, Japanese Medaka, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human LC3B. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_2888317

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

microtubule associated protein 1 light chain 3 beta , ATG8F , LC3B , MAP1A/1BLC3 , MAP1LC3B-a

Cellular Localization

Cytoplasm , Endomembrane system; Lipid-anchor , Cytoplasmic vesicle , autophagosome membrane; Lipid-anchor

Background

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LC3B antibody [GT3612] used in Western Blot (WB). GTX632501

GTX632501 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [GT3612] (GTX632501) diluted at 1:500.

Anti-LC3B antibody [GT3612] used in Western Blot (WB). GTX632501

GTX632501 WB Image

Orthogonal

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [GT3612] (GTX632501) diluted at 1:500.

Anti-LC3B antibody [GT3612] used in Western Blot (WB). GTX632501

GTX632501 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [GT3612] (GTX632501) diluted at 1:500.

Anti-LC3B antibody [GT3612] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632501

GTX632501 ICC/IF Image

LC3B antibody [GT3612] detects LC3B protein at autophagosome by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LC3B protein stained by LC3B antibody [GT3612] (GTX632501) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

Application Reference

Zhang M et al. J Pharmacol Sci 2021; 147 (3) : 234-244 Liraglutide ameliorates diabetes-associated cognitive dysfunction via rescuing autophagic flux.
Application : ICC/IF
Wan C et al. Cell Death Dis 2019; 10 (6) : 392 Silver nanoparticles selectively induce human oncogenic 帠-herpesvirus-related cancer cell death through reactivating viral lytic replication.
Application : WB
Reactivity : Human
Tsai YG et al. Sci Rep 2018; 8 (1) : 12973 Complement regulatory protein CD46 induces autophagy against oxidative stress-mediated apoptosis in normal and asthmatic airway epithelium.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations