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LC3B antibody [HL2798]

Anti-LC3B antibody [HL2798] used in IHC (Paraffin sections) (IHC-P). GTX639686
Anti-LC3B antibody [HL2798] used in IHC (Paraffin sections) (IHC-P). GTX639686
Anti-LC3B antibody [HL2798] used in IHC (Paraffin sections) (IHC-P). GTX639686
Anti-LC3B antibody [HL2798] used in Western Blot (WB). GTX639686
Anti-LC3B antibody [HL2798] used in Western Blot (WB). GTX639686
Anti-LC3B antibody [HL2798] used in Western Blot (WB). GTX639686

Cat. No. GTX639686

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL2798

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide of human LC3B.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

microtubule associated protein 1 light chain 3 beta , ATG8F , LC3B , MAP1A/1BLC3 , MAP1LC3B-a

Cellular Localization

Cytoplasm, cytoskeleton

Background

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-LC3B antibody [HL2798] used in IHC (Paraffin sections) (IHC-P). GTX639686

GTX639686 IHC-P Image

LC3B antibody [HL2798] detects LC3B protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
LC3B stained by LC3B antibody [HL2798] (GTX639686) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LC3B antibody [HL2798] used in IHC (Paraffin sections) (IHC-P). GTX639686

GTX639686 IHC-P Image

LC3B antibody [HL2798] detects LC3B protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
LC3B stained by LC3B antibody [HL2798] (GTX639686) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LC3B antibody [HL2798] used in IHC (Paraffin sections) (IHC-P). GTX639686

GTX639686 IHC-P Image

LC3B antibody [HL2798] detects LC3B protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
LC3B stained by LC3B antibody [HL2798] (GTX639686) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LC3B antibody [HL2798] used in Western Blot (WB). GTX639686

GTX639686 WB Image

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [HL2798] (GTX639686) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LC3B antibody [HL2798] used in Western Blot (WB). GTX639686

GTX639686 WB Image

Untreated (–) and treated (+) PC-12 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [HL2798] (GTX639686) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LC3B antibody [HL2798] used in Western Blot (WB). GTX639686

GTX639686 WB Image

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with LC3B antibody [HL2798] (GTX639686) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations