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LC3B antibody [N1C3]

Anti-LC3B antibody [N1C3] used in Western Blot (WB). GTX116080
Anti-LC3B antibody [N1C3] used in Western Blot (WB). GTX116080
Anti-LC3B antibody [N1C3] used in Western Blot (WB). GTX116080
Anti-LC3B antibody [N1C3] used in Flow cytometry (FACS). GTX116080
Anti-LC3B antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116080
Anti-LC3B antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX116080

Cat. No. GTX116080

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all LC3B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS 1:50-1:200
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

Mouse brain , Rat brain , U87-MG

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Japanese Medaka, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.37 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human LC3B Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10626269

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

microtubule associated protein 1 light chain 3 beta , ATG8F , LC3B , MAP1A/1BLC3 , MAP1LC3B-a

Cellular Localization

Cytoplasm , Endomembrane system; Lipid-anchor , Cytoplasmic vesicle , autophagosome membrane; Lipid-anchor

Background

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LC3B antibody [N1C3] used in Western Blot (WB). GTX116080

GTX116080 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
15% SDS PAGE
GTX116080 diluted at 1:1000

Anti-LC3B antibody [N1C3] used in Western Blot (WB). GTX116080

GTX116080 WB Image

Sample (50 ug of whole cell lysate)
A: Rat brain
12% SDS PAGE
GTX116080 diluted at 1:1000

Anti-LC3B antibody [N1C3] used in Western Blot (WB). GTX116080

GTX116080 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
15% SDS PAGE
GTX116080 diluted at 1:1000

Anti-LC3B antibody [N1C3] used in Flow cytometry (FACS). GTX116080

GTX116080 FACS Image

LC3B antibody [N1C3] (GTX116080) detects LC3B protein by flow cytometry analysis.
Sample: HeLa cell fixed in 4% paraformaldehyde at 4oC for 5 min.
Brown: Unlabelled sample was also used as a control.
Blue: LC3B antibody [N1C3] (GTX116080) dilution: 1:100.
Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-LC3B antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116080

GTX116080 ICC/IF Image

LC3B antibody [N1C3] detects LC3B protein at autophagosome by immunofluorescent analysis.
Samples: Hep G2 cells mock (left) and treated with 3 μM Thapsigargin for 12 hrs (right) were fixed in ice-cold MeOH for 10 min and permeabilized with ice-cold acetone for 1 min.
Green: LC3B protein stained by LC3B antibody [N1C3] (GTX116080) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-LC3B antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX116080

GTX116080 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human Breast carcinoma, using LC3B(GTX116080) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Marino N et al. NPJ Breast Cancer 2020; 6 : 50 Upregulation of lipid metabolism genes in the breast prior to cancer diagnosis.
Application : IHC-P
Reactivity : Human
Li Y et al. ACS Omega 2018; 3 (6) : 6812-6819 Effective Theranostic Cyanine for Imaging of Amyloid Species in Vivo and Cognitive Improvements in Mouse Model.
Yinhui Li et al. ACS OMEGA 2018; 3 (6) : pp 6812–6819 Effective Theranostic Cyanine for Imaging of Amyloid Species in Vivo and Cognitive Improvements in Mouse Model.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169