GeneTex
United States (US)

LC3B antibody

GTX100240_40555_CT_1_WB_19011506_101.jpg
GTX100240_41115_WB_19011506_299.jpg
GTX100240_40555_CT_WB_19011506_965.jpg
GTX100240_41115_WB_R_brain_19011506_993.jpg
GTX100240_41115_WB_M_brain_19011506_235.jpg
GTX100240_41115_IHC_19011506_160.jpg
GTX100240_41115_WB_2_19011506_492.jpg

Cat No. GTX100240

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS , PC-3 , U87-MG , SK-N-SH , Rat brain , mouse brain

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Japanese Medaka, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Full length rat LC3B Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

microtubule-associated proteins 1A/1B light chain 3 antibody, autophagy-related ubiquitin-like modifier LC3 B antibody, MAP1A/MAP1B LC3 B antibody, MAP1A/MAP1B light chain 3 B antibody, MAP1A/1B light chain 3 B antibody, MAP1 light chain 3-like protein 2 antibody, autophagy-related protein LC3 B antibody, microtubule-associated proteins 1A/1B light chain 3B antibody, MGC93422 antibody, Map1lc3 antibody, Mpl3 antibody, zbs559 antibody, Map1lc3b antibody, microtubule-associated protein 1 light chain 3 beta antibody

Cellular Localization

Cytoplasm , Endomembrane system; Lipid-anchor , Cytoplasmic vesicle , autophagosome membrane; Lipid-anchor

Background

may mediate MAPlA and MAPlB microtubule binding activity during development of the nervous system [RGD]

Database

Research Area

DATA IMAGES
GTX100240_40555_CT_1_WB_19011506_101.jpg

GTX100240 WB Image

Sample (50 ug of whole cell lysate)
A: Huh7  
B: virus infected Huh7
12% SDS PAGE
GTX100240 diluted at 1:1000

GTX100240_41115_WB_19011506_299.jpg

GTX100240 WB Image

LC3B antibody detects LC3B protein by western blot analysis.
A. 30 μg U87-MG whole cell lysate/extract
B. 30 μg SK-N-SH whole cell lysate/extract
15 % SDS-PAGE
LC3B antibody (GTX100240) dilution: 1:500

GTX100240_40555_CT_WB_19011506_965.jpg

GTX100240 WB Image

Western blot analysis of LC3 protein in PC-3 cell line. (1) 20ug of whole cell lysate was separated by using Urea gel (6M Urea in 13.5% SDS gel) and detected with GTX100240 unpurified serum diluted at 1:4000. (2) PC-3 cells were cultured with serum-containing medium (A) and serum-free medium (B) for 3 days. 5ug of whole cell lysate was separated by using Urea gel (6M Urea in 13.5% SDS gel) and detected with GTX100240 antibody diluted at 1:5000.

GTX100240_41115_WB_R_brain_19011506_993.jpg

GTX100240 WB Image

LC3B antibody detects LC3B protein by western blot analysis.
A. 50 μg rat brain extract
15 % SDS-PAGE
LC3B antibody (GTX100240) dilution: 1:500

GTX100240_41115_WB_M_brain_19011506_235.jpg

GTX100240 WB Image

LC3B antibody detects LC3B protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
15 % SDS-PAGE
LC3B antibody (GTX100240) dilution: 1:500

GTX100240_41115_IHC_19011506_160.jpg

GTX100240 IHC-P Image

LC3B antibody detects LC3B protein at cytosol on human gastric carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded gastric carcinoma.
LC3B antibody (GTX100240) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100240_41115_WB_2_19011506_492.jpg

GTX100240 WB Image

LC3B antibody detects LC3B protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15 % SDS-PAGE, and blotted with LC3B antibody (GTX100240) diluted by 1:500

REVIEW

LC3B antibody Cat No. GTX100240

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 03-Jun-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : PANC-1 cells,AS-PC cells
Amount used : 30μg
Blocking : 5% TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf