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LC3B antibody

Anti-LC3B antibody used in IHC (Paraffin sections) (IHC-P). GTX31899
Anti-LC3B antibody used in Western Blot (WB). GTX31899
Anti-LC3B antibody used in IHC (Paraffin sections) (IHC-P). GTX31899

Cat. No. GTX31899

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all LC3B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , Human Brain Tissue Lysate(GTX27918)

Product Note

Multiple isoforms are known to exist.LC3B antibody is predicted to not cross-react with LC3A or LC3C.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

LC3B antibody was raised against an 18 amino acid peptide near the amino terminus of human LC3B.The immunogen is located within the first 50 amino acids of LC3B.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

microtubule associated protein 1 light chain 3 beta , ATG8F , LC3B , MAP1A/1BLC3 , MAP1LC3B-a

Cellular Localization

Cytoplasm, cytoskeleton

Background

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-LC3B antibody used in IHC (Paraffin sections) (IHC-P). GTX31899

GTX31899 IHC-P Image

IHC-P analysis of human brain tissue using GTX31899 LC3B antibody.
Working concentration : 20 μg/ml

Anti-LC3B antibody used in Western Blot (WB). GTX31899

GTX31899 WB Image

WB analysis of human brain tissue lysate using GTX31899 LC3B antibody.
Working concentration : 1 μg/ml

Anti-LC3B antibody used in IHC (Paraffin sections) (IHC-P). GTX31899

GTX31899 IHC-P Image

IHC-P analysis of human brain tissue using GTX31899 LC3B antibody.
Working concentration : 5 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399