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LC3B antibody

Anti-LC3B antibody used in IHC (Paraffin sections) (IHC-P). GTX82986
Anti-LC3B antibody used in Western Blot (WB). GTX82986
Anti-LC3B antibody used in IHC (Paraffin sections) (IHC-P). GTX82986
Anti-LC3B antibody used in Western Blot (WB). GTX82986
Anti-LC3B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82986
Anti-LC3B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82986
Anti-LC3B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82986
Anti-LC3B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82986
Anti-LC3B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82986

Cat No. GTX82986

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, EM

Reactivity

Human, Mouse, Rat, Zebrafish, Bovine, Dog, Pig, Primate
Package
100 μl ($389)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 0.1 - 2 μg/ml
IHC-P 1:200 - 1:400
IHC-Fr 1:400
EM Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.02% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide made to the N-terminal region of the human LC3B protein. [UniProt# Q9GZQ8]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_848127

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

microtubule associated protein 1 light chain 3 beta , ATG8F , LC3B , MAP1A/1BLC3 , MAP1LC3B-a

Cellular Localization

Cytoplasm, cytoskeleton

Background

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LC3B antibody used in IHC (Paraffin sections) (IHC-P). GTX82986

GTX82986 IHC-P Image

IHC-P analysis of normal brain and gliobastoma multiform tissue using GTX82986 LC3B antibody.

Anti-LC3B antibody used in Western Blot (WB). GTX82986

GTX82986 WB Image

WB analysis of Neuro2a cell lysate using GTX82986 LC3B antibody.

Anti-LC3B antibody used in IHC (Paraffin sections) (IHC-P). GTX82986

GTX82986 IHC-P Image

IHC-P analysis of temozolomide-treated U87-MG xenografts tissue using GTX82986 LC3B antibody.

Anti-LC3B antibody used in Western Blot (WB). GTX82986

GTX82986 WB Image

WB analysis of arsenic trioxide treated U87-MG cell lysate using GTX82986 LC3B antibody.

Anti-LC3B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82986

GTX82986 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX82986 LC3B antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1 μg/ml

Anti-LC3B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82986

GTX82986 ICC/IF Image

ICC/IF analysis of C2-ceramide treated U373-MG cells using GTX82986 LC3B antibody.

Anti-LC3B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82986

GTX82986 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX82986 LC3B antibody.
Green : primary antibody
Red : Actin
Blue : DAPI
Dilution : 1:5

Anti-LC3B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82986

GTX82986 ICC/IF Image

ICC/IF analysis of temozolomide treated U373-MG cells using GTX82986 LC3B antibody.

Anti-LC3B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82986

GTX82986 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX82986 LC3B antibody.
Red : primary antibody
Blue : DAPI

REFERENCE

REVIEW

LC3B antibody Cat No. GTX82986

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2016.PMID: 27537685
Application Reference
SDS
PBS.pdf
Sodium Azide.pdf