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LC3C antibody

Anti-LC3C antibody used in Western Blot (WB). GTX32125
Anti-LC3C antibody used in IHC (Paraffin sections) (IHC-P). GTX32125
Anti-LC3C antibody used in IHC (Paraffin sections) (IHC-P). GTX32125

Cat. No. GTX32125

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all LC3C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , Human Brain Tissue Lysate(GTX27918)

Product Note

Multiple isoforms are known to exist. This antibody is predicted to not cross-react with MAP1LC3A or MAP1LC3B.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

LC3C antibody was raised against an 18 amino acid peptide near the carboxy terminus of human LC3C .The immunogen is located within the last 50 amino acids of LC3C .

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

microtubule associated protein 1 light chain 3 gamma , ATG8J , LC3C

Cellular Localization

Cytoplasm, cytoskeleton

Background

Autophagy is a highly regulated bulk degradation process that plays an important role in cellular maintenance and development. MAP1LC3C is an ortholog of the yeast autophagosome protein Atg8 (He et al., 2003 [PubMed 12740394]).[supplied by OMIM, Nov 2010]

Database

Research Area

DATA IMAGES

Anti-LC3C antibody used in Western Blot (WB). GTX32125

GTX32125 WB Image

WB analysis of human brain tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX32125 LC3C antibody.
Working concentration : 1 μg/ml

Anti-LC3C antibody used in IHC (Paraffin sections) (IHC-P). GTX32125

GTX32125 IHC-P Image

IHC-P analysis of human brain tissue using GTX32125 LC3C antibody.
Working concentration : 5 μg/ml

Anti-LC3C antibody used in IHC (Paraffin sections) (IHC-P). GTX32125

GTX32125 IHC-P Image

IHC-P analysis of human brain tissue using GTX32125 LC3C antibody.
Working concentration : 20 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399