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LCMT2 antibody

Anti-LCMT2 antibody used in Western Blot (WB). GTX122895
Anti-LCMT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX122895
Anti-LCMT2 antibody used in Western Blot (WB). GTX122895
Anti-LCMT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX122895

Cat. No. GTX122895

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all LCMT2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Positive Control

HepG2 , Mouse brain

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.03 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LCMT2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11172194

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

leucine carboxyl methyltransferase 2 , PPM2 , TYW4

Background

The protein encoded by this intronless gene belongs to the highly variable methyltransferase superfamily. This gene is the inferred homolog of the Saccharomyces cerevisiae carboxymethyltransferase gene PPM2 that is essential for the synthesis of the hypermodified guanosine Wybutosine (yW). [provided by RefSeq]

Database

DATA IMAGES

Anti-LCMT2 antibody used in Western Blot (WB). GTX122895

GTX122895 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
7.5% SDS PAGE
GTX122895 diluted at 1:1000

Anti-LCMT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX122895

GTX122895 IHC-P Image

LCMT2 antibody detects LCMT2 protein at cytosol and nucleus on SkHep1 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded SkHep1 xenograft.
LCMT2 antibody (GTX122895) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-LCMT2 antibody used in Western Blot (WB). GTX122895

GTX122895 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX122895 diluted at 1:1000

Anti-LCMT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX122895

GTX122895 IHC-P Image

LCMT2 antibody detects LCMT2 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
LCMT2 antibody (GTX122895) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169