LDB1 antibody [N2C3]

Cat. No. GTX104531

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all LDB1 products

Datasheet
Document

View product citations for antibody GTX104531 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

Mouse brain , HeLa

Predict Reactivity

Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LDB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950805

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

LIM domain binding 1 , CLIM-2 , CLIM2 , LDB-1 , NLI

Cellular Localization

Nucleus

Background

Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. May play a role in the development of motor neurons. Acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. Acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state.

Database

Gene ID: 8861 LDB1
UniProt: Q86U70 LDB1

Research Area

DATA IMAGES

GTX104531 WB Image

Wild-type (WT) and LDB1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDB1 antibody [N2C3] (GTX104531) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX104531 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDB1 antibody [N2C3] (GTX104531) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104531 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX104531 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104531 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 Xenograft, using LDB1(GTX104531) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104531 ICC/IF Image

LDB1 antibody [N2C3] detects LDB1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LDB1 protein stained by LDB1 antibody [N2C3] (GTX104531) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX104531 IHC-P Image

LDB1 antibody [N2C3] detects LDB1 protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
LDB1 antibody [N2C3] (GTX104531) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104531 IP Image

LDB1antibody immunoprecipitates LDB1 protein in IP experiments.
IP Sample: 1000 μg 293T whole cell lysate/extract
A. 20 μg 293T whole cell lysate/extract
B. Control with 2.5 μg of preimmune rabbit IgG
C. Immunoprecipitation of LDB1 protein by 2.5 μg of LDB1 antibody (GTX104531)
12% SDS-PAGE
The immunoprecipitated LDB1 protein was detected by LDB1antibody (GTX104531) diluted at 1:1000.
EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX104531 IHC-P Image

LDB1 antibody [N2C3] detects LDB1 protein at nucleus on rat middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat middle brain.
LDB1 antibody [N2C3] (GTX104531) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty

LDB1 antibody [N2C3]

Cat. No. GTX104531

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Positive Control

Predict Reactivity

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX104531 WB Image

GTX104531 WB Image

GTX104531 WB Image

GTX104531 IHC-P Image

GTX104531 ICC/IF Image

GTX104531 IHC-P Image

GTX104531 IP Image

GTX104531 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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