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LDB1 antibody [N2C3]

KO/KD
Anti-LDB1 antibody [N2C3] used in Western Blot (WB). GTX104531
Anti-LDB1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104531
Anti-LDB1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104531
Anti-LDB1 antibody [N2C3] used in Western Blot (WB). GTX104531
Anti-LDB1 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104531
Anti-LDB1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104531
Anti-LDB1 antibody [N2C3] used in Immunoprecipitation (IP). GTX104531
Anti-LDB1 antibody [N2C3] used in Western Blot (WB). GTX104531

Cat No. GTX104531

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all LDB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Mouse brain

Predict Reactivity

Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LDB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950805

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

LIM domain binding 1 , CLIM-2 , CLIM2 , LDB-1 , NLI

Cellular Localization

Nucleus

Background

Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. May play a role in the development of motor neurons. Acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. Acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state.

Database

Research Area

DATA IMAGES

Anti-LDB1 antibody [N2C3] used in Western Blot (WB). GTX104531

GTX104531 WB Image

KO/KD

Wild-type (WT) and LDB1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDB1 antibody [N2C3] (GTX104531) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-LDB1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104531

GTX104531 IHC-P Image

LDB1 antibody [N2C3] detects LDB1 protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
LDB1 antibody [N2C3] (GTX104531) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-LDB1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104531

GTX104531 IHC-P Image

LDB1 antibody [N2C3] detects LDB1 protein at nucleus on rat middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat middle brain.
LDB1 antibody [N2C3] (GTX104531) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-LDB1 antibody [N2C3] used in Western Blot (WB). GTX104531

GTX104531 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX104531 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LDB1 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104531

GTX104531 ICC/IF Image

LDB1 antibody [N2C3] detects LDB1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LDB1 protein stained by LDB1 antibody [N2C3] (GTX104531) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-LDB1 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104531

GTX104531 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 Xenograft, using LDB1(GTX104531) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-LDB1 antibody [N2C3] used in Immunoprecipitation (IP). GTX104531

GTX104531 IP Image

LDB1antibody immunoprecipitates LDB1 protein in IP experiments.
IP Sample: 1000 μg 293T whole cell lysate/extract
A. 20 μg 293T whole cell lysate/extract
B. Control with 2.5 μg of preimmune rabbit IgG
C. Immunoprecipitation of LDB1 protein by 2.5 μg of LDB1 antibody (GTX104531)
12% SDS-PAGE
The immunoprecipitated LDB1 protein was detected by LDB1antibody (GTX104531) diluted at 1:1000.
EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-LDB1 antibody [N2C3] used in Western Blot (WB). GTX104531

GTX104531 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDB1 antibody [N2C3] (GTX104531) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169