GeneTex
United States (US)

LDB1 antibody [N2C3]

KO/KD
GTX104531_41766_20181109_WB_KO_watermark_19011519_940.jpg
GTX104531_41073_IHC_M_19011506_425.jpg
GTX104531_41073_IHC_R_19011506_672.jpg
GTX104531_40079_WB_M_brain_19011506_140.jpg
GTX104531_40709_20150513_IFA_19011506_559.jpg
GTX104531_IHC_19011506_892.jpg
GTX104531_40079_IP_19011506_386.jpg
GTX104531_41766_WB_19011506_433.jpg

Cat No. GTX104531

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Mouse brain

Predict Reactivity

Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LDB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CLIM2 antibody, NLI antibody, LDB1 antibody, CLIM-2 antibody, hLdb1 antibody, LDB-1 antibody, LIM domain-binding factor-1 antibody, nuclear LIM interactor antibody, LIM domain-binding factor CLIM2 antibody, carboxyl-terminal LIM domain-binding protein 2 antibody, carboxy terminal LIM domain protein 2 antibody, LIM domain-binding protein 1 antibody, LIM domain binding 1 antibody

Cellular Localization

Nucleus

Background

Binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. May play a role in the development of motor neurons. Acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. Acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state.

Database

Research Area

DATA IMAGES
GTX104531_41766_20181109_WB_KO_watermark_19011519_940.jpg

GTX104531 WB Image

KO/KD

Wild-type (WT) and LDB1 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDB1 antibody [N2C3] (GTX104531) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
CRISPR knockout cell lines generated by www.edigene.com

GTX104531_41073_IHC_M_19011506_425.jpg

GTX104531 IHC-P Image

LDB1 antibody [N2C3] detects LDB1 protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
LDB1 antibody [N2C3] (GTX104531) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104531_41073_IHC_R_19011506_672.jpg

GTX104531 IHC-P Image

LDB1 antibody [N2C3] detects LDB1 protein at nucleus on rat middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat middle brain.
LDB1 antibody [N2C3] (GTX104531) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104531_40079_WB_M_brain_19011506_140.jpg

GTX104531 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX104531 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104531_40709_20150513_IFA_19011506_559.jpg

GTX104531 ICC/IF Image

LDB1 antibody [N2C3] detects LDB1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LDB1 protein stained by LDB1 antibody [N2C3] (GTX104531) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX104531_IHC_19011506_892.jpg

GTX104531 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 Xenograft, using LDB1(GTX104531) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104531_40079_IP_19011506_386.jpg

GTX104531 IP Image

LDB1antibody immunoprecipitates LDB1 protein in IP experiments.
IP Sample: 1000 µg 293T whole cell lysate/extract
A. 20 μg 293T whole cell lysate/extract
B. Control with 2.5 μg of preimmune rabbit IgG
C. Immunoprecipitation of LDB1 protein by 2.5 μg of LDB1 antibody (GTX104531)
12% SDS-PAGE
The immunoprecipitated LDB1 protein was detected by LDB1antibody (GTX104531) diluted at 1:1000.
EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX104531_41766_WB_19011506_433.jpg

GTX104531 WB Image

LDB1 antibody detects LDB1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDB1 antibody (GTX104531) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169