Wild-type (WT) and LDH-B knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDH-B antibody (GTX101747) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed HeLa, using LDH-B(GTX101747) antibody at 1:50 dilution.

LDH-B antibody detects LDHB protein at cytosol on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
LDH-B antibody (GTX101747) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDH-B antibody (GTX101747) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

LDH-B antibody detects LDH-B protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
LDH-B stained by LDH-B antibody (GTX101747) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDH-B antibody (GTX101747) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal BMC Genomics in 2015. PMID: 25652794