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LDH-B antibody

Anti-LDH-B antibody used in Western Blot (WB). GTX101747
Anti-LDH-B antibody used in Western Blot (WB). GTX101747
Anti-LDH-B antibody used in Western Blot (WB). GTX101747
Anti-LDH-B antibody used in IHC (Paraffin sections) (IHC-P). GTX101747
Anti-LDH-B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101747

Cat No. GTX101747

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

293T , U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse brain , rat brain

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LDH-B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

lactate dehydrogenase B , HEL-S-281 , LDH-B , LDH-H , LDHBD , TRG-5

Cellular Localization

Cytoplasm

Background

This gene encodes an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on the X chromosome and on chromosome. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Database

DATA IMAGES
Anti-LDH-B antibody used in Western Blot (WB). GTX101747

GTX101747 WB Image

Wild-type (WT) and LDH-B knockout (KO) 293T cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDH-B antibody (GTX101747) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LDH-B antibody used in Western Blot (WB). GTX101747

GTX101747 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDH-B antibody (GTX101747) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LDH-B antibody used in Western Blot (WB). GTX101747

GTX101747 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with LDH-B antibody (GTX101747) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LDH-B antibody used in IHC (Paraffin sections) (IHC-P). GTX101747

GTX101747 IHC-P Image

LDH-B antibody detects LDHB protein at cytosol on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
LDH-B antibody (GTX101747) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-LDH-B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101747

GTX101747 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using LDH-B(GTX101747) antibody at 1:50 dilution.

REFERENCE
REVIEW

LDH-B antibody Cat No. GTX101747

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 29-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MCF7 cells
Blocking : 5%milk in TBST
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169