LDHA antibody [2D11]

Cat. No. GTX84227

Host	Mouse
Clonality	Monoclonal
Clone Name	2D11
Isotype	IgG1
Application	WB, IHC-P, IP
Reactivity	Human, Mouse, Rat, Dog, Monkey
Package	100 μl ($519)

See all LDHA products

Datasheet
Document

View product citations for antibody GTX84227 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500
IHC-P	1:50
IP	2ug/500ul

Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human LDHA (NP_005557) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lactate dehydrogenase A , GSD11 , HEL-S-133P , LDHM , PIG19

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq]

Database

Gene ID: 3939 LDHA
UniProt: P00338 LDHA

Research Area

DATA IMAGES

GTX84227 WB Image

WB analysis of HEK293T cells transfected with LDHA plasmid (Right) or empty vector (Left) for 48 hrs using GTX84227 LDHA antibody [2D11].
Loading : 5 ug per lane

GTX84227 IHC-P Image

IHC-P analysis of human thyroid carcinoma tissue using GTX84227 LDHA antibody [2D11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84227 IHC-P Image

IHC-P analysis of human bladder carcinoma tissue using GTX84227 LDHA antibody [2D11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84227 IHC-P Image

IHC-P analysis of human pancreas carcinoma tissue using GTX84227 LDHA antibody [2D11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84227 IHC-P Image

IHC-P analysis of human endometrium adenocarcinoma tissue using GTX84227 LDHA antibody [2D11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84227 IP Image

IP analysis of DDDDK tagged LDHA overexpressed HEK293T lysate using GTX84227 LDHA antibody [2D11].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

GTX84227 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84227 LDHA antibody [2D11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84227 IHC-P Image

IHC-P analysis of human breast adenocarcinoma tissue using GTX84227 LDHA antibody [2D11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX84227 WB Image

WB analysis of various cell lines using GTX84227 LDHA antibody [2D11].
Loading : 35 ug per lane
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty

LDHA antibody [2D11]

Cat. No. GTX84227

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX84227 WB Image

GTX84227 IHC-P Image

GTX84227 IHC-P Image

GTX84227 IHC-P Image

GTX84227 IHC-P Image

GTX84227 IP Image

GTX84227 IHC-P Image

GTX84227 IHC-P Image

GTX84227 WB Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.