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LDHA antibody [AT1A4]

Anti-LDHA antibody [AT1A4] used in Western Blot (WB). GTX50001
Anti-LDHA antibody [AT1A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50001

Cat No. GTX50001

Host

Mouse

Clonality

Monoclonal

Clone Name

AT1A4

Isotype

IgG2b

Application

WB, ICC/IF, FACS, ELISA

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

In Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human LDHA (1-332aa) purified from E.coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Lactate Dehydrogenase A , Gsd11 , Hel-S-133P , Ldhm , Pig19 , Ldha

Background

Lactate through lactate dehydrogenase A (LDHA), which is encoded by a target gene of c-Myc and hypoxia-inducible factor (HIF-1). LDHA expression is repressed by SugR in the absence of sugar. Reduction of LDHA causes bioenergetic and oxidative stress leading to cell death. LDHA (LDH-5, M-LDH, or A4), which is the predominant form in skeletal muscle, kinetically favors the conversion of pyruvate to lactate. Therefore, LDH-A is an attractive target for cancer therapy since its expression is largely relegated to skeletal muscle.

Database

Research Area

DATA IMAGES

Anti-LDHA antibody [AT1A4] used in Western Blot (WB). GTX50001

GTX50001 WB Image

Cell lysates of HeLa (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human LDHA (1:8000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Anti-LDHA antibody [AT1A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50001

GTX50001 ICC/IF Image

Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human LDHA antibody (1:500) with Texas Red (Red).

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf