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LDHA antibody [AT1A4]

Anti-LDHA antibody [AT1A4] used in Western Blot (WB). GTX50001
Anti-LDHA antibody [AT1A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50001

Cat. No. GTX50001

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT1A4

Isotype

 IgG2b

Application

 WB, ICC/IF, FACS, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all LDHA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human LDHA (1-332aa) purified from E.coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lactate dehydrogenase A , GSD11 , HEL-S-133P , LDHM , PIG19

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008]

Database

Research Area

DATA IMAGES

Anti-LDHA antibody [AT1A4] used in Western Blot (WB). GTX50001

GTX50001 WB Image

Cell lysates of HeLa (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human LDHA (1:8000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Anti-LDHA antibody [AT1A4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50001

GTX50001 ICC/IF Image

Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human LDHA antibody (1:500) with Texas Red (Red).

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399