GeneTex
United States (US)

LDHA antibody

GTX32701_20180530_WB_18121410_530.jpg
GTX32701_20180530_ICCIF_18121410_770.jpg

Cat No. GTX32701

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:10 - 1:100
Not tested in other applications.

Calculated MW

37 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-332 of human LDHA (NP_005557.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Lactate Dehydrogenase A,Gsd11,Hel-S-133P,Ldhm,Pig19,Ldha

Background

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008]

Database

Research Area

DATA IMAGES
GTX32701_20180530_WB_18121410_530.jpg

GTX32701 WB Image

WB analysis of various samples using GTX32701 LDHA antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX32701_20180530_ICCIF_18121410_770.jpg

GTX32701 ICC/IF Image

ICC/IF analysis of A549 cell using GTX32701 LDHA antibody.
Blue : DAPI