GeneTex
United States (US)

LDL Receptor antibody [15C8]

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Cat No. GTX16897

Host Mouse
Clonality Monoclonal
Clone Name 15C8
Isotype IgG2a
Application WB, ICC/IF, IHC-Fr, FACS, IP, ELISA
Reactivity Human, Bovine, Cow
APPLICATION

Application Note

For ELISA: Use at a concentration of 0.5-1 μg/ml. For FACS: Use 5μg (undiluted) to label 10^6 clells - Incubate for 30min at 4oC. For ICC: Use at a dilution of 1-5 μg/ml. For IHC-Fr: Use at a dilution of 1-5 μg/ml. For IP: Use at a dilution of 1-2 μg/ml. For WB: Use at a dilution of 1-5 μg/ml. Predicted molecular weight: 160 kDa. Include 2mM Ca+2 with the antibody and avoid EDTA. Product GTX16897 has been reported to bind best to the unreduced LDL-R. Optimal dilutions/concentrations should be determined by the researcher.

Positive Control

Liver sections, HT1080 cells, HepG2 cells - Negative controls: HepG2 cells and normal skin
PROPERTIES

Form

Liquid

Buffer

0.05M sodium phosphate containing 0.2% gelatin and 0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Bovine

Immunogen

A trypic digest of low density lipoprotein receptor from bovine

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Low Density Lipoprotein Receptor,Ldlr

Cellular Localization

Cell Membrane and Cytoplasmic

Background

The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia.

Research Area

DATA IMAGES
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GTX16897 ICC/IF Image

ICC: Human LDL in HepG2 cells fixed in methanol.