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LDL Receptor antibody [15C8]

Anti-LDL Receptor antibody [15C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16897

Cat. No. GTX16897

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 15C8

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-Fr, FACS, IP, ELISA

Reactivity

 Human, Bovine
Package
50 μg ($399)
See all LDL Receptor products

APPLICATION

Application Note

For ELISA: Use at a concentration of 0.5-1 μg/ml. For FACS: Use 5μg (undiluted) to label 10^6 clells - Incubate for 30min at 4oC. For ICC: Use at a dilution of 1-5 μg/ml. For IHC-Fr: Use at a dilution of 1-5 μg/ml. For IP: Use at a dilution of 1-2 μg/ml. For WB: Use at a dilution of 1-5 μg/ml. Predicted molecular weight: 160 kDa. Include 2mM Ca+2 with the antibody and avoid EDTA. Product GTX16897 has been reported to bind best to the unreduced LDL-R. Optimal dilutions/concentrations should be determined by the researcher.

Positive Control

Liver sections, HT1080 cells, HepG2 cells - Negative controls: HepG2 cells and normal skin

PROPERTIES

Form

Liquid

Buffer

50mM Sodium Phosphate, 0.2% Gelatin

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Bovine

Immunogen

A trypic digest of low density lipoprotein receptor from bovine

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Low Density Lipoprotein Receptor , Ldlr

Cellular Localization

Cell Membrane and Cytoplasmic

Background

The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia.

Research Area

DATA IMAGES

Anti-LDL Receptor antibody [15C8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16897

GTX16897 ICC/IF Image

ICC: Human LDL in HepG2 cells fixed in methanol.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399