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LDL Receptor antibody

Anti-LDL Receptor antibody used in Western Blot (WB). GTX132860

Cat No. GTX132860

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

95 kDa. ( Note )

Positive Control

HepG2 , HepG2 (3μM Thapsigargin treatment for 24 hr)

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Human LDL Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

low density lipoprotein receptor,FH,FHC,LDLCQ2

Cellular Localization

Membrane; Single-pass type I membrane protein , Membrane , clathrin-coated pit; Single-pass type I membrane protein

Background

The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-LDL Receptor antibody used in Western Blot (WB). GTX132860

GTX132860 WB Image

Untreated (–) and treated (+) HepG2 whole cell extracts (60 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LDL Receptor antibody (GTX132860) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 20089850

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169