LEF1 antibody [HL5728]

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LEF1 antibody [HL5728] (GTX646679) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

LEF1 antibody [HL5728] detects LEF1 protein by immunofluorescent analysis.
Sample: SW480 cells were fixed in ice-cold MeOH for 5 min.
Green: LEF1 stained by LEF1 antibody [HL5728] (GTX646679) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LEF1 antibody [HL5728] (GTX646679) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate. Corresponding RNA expression data are based on NCBI database.

LEF1 antibody [HL5728] detects LEF1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
LEF1 stained by LEF1 antibody [HL5728] (GTX646679) diluted at 1:200.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LEF1 antibody [HL5728] (GTX646679) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA expression data are based on Human Protein Atlas program.