LEF1 antibody [HL5729]

LEF1 antibody [HL5729] detects LEF1 protein by immunofluorescent analysis.
Sample: SW480 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LEF1 stained by LEF1 antibody [HL5729] (GTX646680) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield™ with DAPI (GTX30920).

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with LEF1 antibody [HL5729] (GTX646680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.