APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-3μg/ml |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89284-PEP).
Predict Reactivity
Mouse, Rat, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-QHEQRKEQEPKRPH, from the internal region of the protein sequence according to NP_057353.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
lymphoid enhancer binding factor 1 , LEF-1 , TCF10 , TCF1ALPHA , TCF7L3
Cellular Localization
Nucleus
Background
This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
Database
Research Area
DATA IMAGES
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GTX89284 WB Image
WB analysis of K562 nuclear extracts using GTX89284 LEF1 antibody, Internal pre-incubated with (Lane B) or without (Lane A) immunogen peptide.
Dilution : 2μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89284 ICC/IF Image
ICC/IF analysis of PFA-fixed Jurkat cells using GTX89284 LEF1 antibody, Internal. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue).
Negative control (-ve) : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml
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GTX89284 ICC/IF Image
ICC/IF analysis of PFA-fixed U2OS cells using GTX89284 LEF1 antibody, Internal. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue).
Negative control (-ve) : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml
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GTX89284 FACS Image
FACS analysis of PFA-fixed Jurkat cells using GTX89284 LEF1 antibody, Internal.
Blue : Primary antibody
Black : Unimmunized goat IgG
Permeabilization : 0.5% Triton
Dilution : 10μg/ml
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REFERENCE
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REVIEW
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