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LEF1 antibody

Anti-LEF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX129186
Anti-LEF1 antibody used in Western Blot (WB). GTX129186
Anti-LEF1 antibody used in Western Blot (WB). GTX129186
Anti-LEF1 antibody used in Western Blot (WB). GTX129186

Cat. No. GTX129186

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

Jurkat , Juarkat_NE

Product Note

Knockdown/Knockout validation was supported by references (PMID:35801925)

Predict Reactivity

Mouse, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human LEF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885917

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lymphoid enhancer binding factor 1 , LEF-1 , TCF10 , TCF1ALPHA , TCF7L3

Cellular Localization

Nucleus

Background

LEF1 is a nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA; MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1; MIM 163905) (Waterman et al., 1991 [PubMed 2010090]; van Genderen et al., 1994 [PubMed 7958926]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-LEF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX129186

GTX129186 IHC-P Image

LEF1 antibody detects LEF1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
LEF1 stained by LEF1 antibody (GTX129186) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LEF1 antibody used in Western Blot (WB). GTX129186

GTX129186 WB Image

LEF1 antibody detects LEF1 protein by western blot analysis. Jurkat whole cell extracts and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LEF1 antibody (GTX129186) diluted by 1:1000.

Anti-LEF1 antibody used in Western Blot (WB). GTX129186

GTX129186 WB Image

The data was published in the journal Arch Dermatol Res in 2019.PMID: 31006055

Anti-LEF1 antibody used in Western Blot (WB). GTX129186

GTX129186 WB Image

The data was published in the journal Int J Mol Sci in 2020.PMID: 32150830

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169