GeneTex
United States (US)

LEF1 antibody

GTX37387_20190129_IHC-P_4_19012901_212.jpg
GTX37387_20190129_IHC-P_2_19012901_621.jpg
GTX37387_20190129_IHC-P_1_19012901_252.jpg
GTX37387_20190129_IHC-P_3_19012901_522.jpg
GTX37387_20190129_IHC-P_19012901_297.jpg

Cat No. GTX37387

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

P-ELISA:1:500-1000, WB:1:100-500, IHC-P:1:100-500 (based on 0.5 mg/ml)

Calculated MW

44 kDa. ( Note )

Predict Reactivity

Bovine, Dog, Chicken, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.01M TBS pH7.4, 0.5% BSA, 0.015% ProClin™ 300, 25% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide derived between 341-389 amino acids of human LEF1

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Lymphoid Enhancer Binding Factor 1,Lef-1,Tcf10,Tcf1Alpha,Tcf7L3,Lef1

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Database

Research Area

DATA IMAGES
GTX37387_20190129_IHC-P_4_19012901_212.jpg

GTX37387 IHC-P Image

IHC-P analysis of rat brain tissue using GTX37387 LEF1 antibody.
Fixation : 4% PFA
Antigen retireval : boiling with citrate buffer
Dilution : 1:200

GTX37387_20190129_IHC-P_2_19012901_621.jpg

GTX37387 IHC-P Image

IHC-P analysis of mouse skin tissue using GTX37387 LEF1 antibody.

GTX37387_20190129_IHC-P_1_19012901_252.jpg

GTX37387 IHC-P Image

IHC-P analysis of rat liver tissue using GTX37387 LEF1 antibody.
Dilution : 1:300

GTX37387_20190129_IHC-P_3_19012901_522.jpg

GTX37387 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX37387 LEF1 antibody.

GTX37387_20190129_IHC-P_19012901_297.jpg

GTX37387 IHC-P Image

IHC-P analysis of mouse tissue using GTX37387 LEF1 antibody.
Dilution : 1:200