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LEFTY2 antibody [7C5G1H6H10]

Anti-LEFTY2 antibody [7C5G1H6H10] used in Western Blot (WB). GTX48643
Anti-LEFTY2 antibody [7C5G1H6H10] used in Western Blot (WB). GTX48643

Cat. No. GTX48643

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7C5G1H6H10

Isotype

 IgG2a

Application

 WB, ELISA

Reactivity

 Human
Package
50 μg ($399)
See all LEFTY2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ELISA 1:5000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant form of 6X His-tagged human LEFTY.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

left-right determination factor 2 , EBAF , LEFTA , LEFTYA , TGFB4

Cellular Localization

Secreted

Background

During vertebrate embryogenesis, a left-right axis is established. Secreted growth factors of the TGF-beta family, including gene products derived from nodal, lefty-1 and lefty-2, play crucial roles in establishing left-right asymmetries. TGF-beta (Transforming growth factor-beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF-beta actions are directed by ligand-induced activation of TGF-beta receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF-beta superfamily, inhibits TGF-beta signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF-beta receptor. Lefty also inhibits events downstream from R-Smad phosphorylation. Lefty provides a repressed state of TGF-beta-responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF-beta superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (42 Kda) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28kDa form induces MAPK activity.

Database

Research Area

DATA IMAGES

Anti-LEFTY2 antibody [7C5G1H6H10] used in Western Blot (WB). GTX48643

GTX48643 WB Image

WB analysis of transfected (lane 2) and non-transfected (lane 2) samples using GTX48643 LEFTY2 antibody [7C5G1H6H10].
Loading : 1 μg
Dilution : 1:2000

Anti-LEFTY2 antibody [7C5G1H6H10] used in Western Blot (WB). GTX48643

GTX48643 WB Image

Mab anti-Human LEFTY antibody (clone 7C5G1H6H10) is shown to detect by western blot partially purified recombinant 6X His tagged human LEFTY. Detection occurs after 1.0 μg of protein is loaded in each lane. The blot was incubated with a 1:2,000 dilution of Mab anti-Human LEFTY at room temperature for 30 min followed by detection using IRDye™800 labeled Goat anti-Mouse IgG [H&L] diluted 1:1,000. Lane 1 contains an unrelated 6X His tagged protein and shows that the antibody does not recognize the epitope tag. Lane 2 contains partially purified recombinant human LEFTY. The antibody may be used to detect endogenous human LEFTY. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.

REFERENCE

Application Reference

Pang-Yen Liu et al. Front Pharmacol 2021; 12 : 724515 The Role of HSP70 in the Protective Effects of NVP-AUY922 on Multiple Organ Dysfunction Syndrome in Endotoxemic Rats.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399