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LGR5 antibody [GT11312]

Anti-LGR5 antibody [GT11312] used in Western Blot (WB). GTX631935

Cat No. GTX631935

Host Mouse
Clonality Monoclonal
Clone Name GT11312
Isotype IgG1
Application WB
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000

Note :

WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.

Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

unboiled Myc-tagged hLGR5-transfected 293T cells

Specificity/Sensitivity

Orthogonal validation was supported by references (PMID:30224759)

Predict Reactivity

Rat, Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LGR5. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Leucine Rich Repeat Containing G Protein-Coupled Receptor 5,Fex,Gpr49,Gpr67,Grp49,Hg38,Lgr5

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Orphan receptor. Stem cell marker of the intestinal epithelium and the hair follicule. Target gene of Wnt signaling.

Database

Research Area

DATA IMAGES
Anti-LGR5 antibody [GT11312] used in Western Blot (WB). GTX631935

GTX631935 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LGR5 antibody [GT11312] (GTX631935) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169