*Optimal dilutions/concentrations should be determined by the researcher.
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.
Not tested in other applications.
unboiled Myc-tagged hLGR5-transfected 293T cells
Orthogonal validation was supported by references (PMID:30224759)
Rat, Bovine, Pig(>80% identity)
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1.3 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human LGR5. The exact sequence is proprietary.
Affinity purified by Protein G.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Leucine Rich Repeat Containing G Protein-Coupled Receptor 5,Fex,Gpr49,Gpr67,Grp49,Hg38,Lgr5
Cell membrane; Multi-pass membrane protein
Orphan receptor. Stem cell marker of the intestinal epithelium and the hair follicule. Target gene of Wnt signaling.