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LGR5 antibody

Anti-LGR5 antibody used in IHC (Paraffin sections) (IHC-P). GTX04555
Anti-LGR5 antibody used in IHC (Paraffin sections) (IHC-P). GTX04555
Anti-LGR5 antibody used in IHC (Paraffin sections) (IHC-P). GTX04555
Anti-LGR5 antibody used in IHC (Paraffin sections) (IHC-P). GTX04555
Anti-LGR5 antibody used in IHC (Paraffin sections) (IHC-P). GTX04555
Anti-LGR5 antibody used in Western Blot (WB). GTX04555
Anti-LGR5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04555
Anti-LGR5 antibody used in IHC (Paraffin sections) (IHC-P). GTX04555
Anti-LGR5 antibody used in IHC (Paraffin sections) (IHC-P). GTX04555

Cat. No. GTX04555

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all LGR5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Predict Reactivity

Sheep, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human LGR5(Accession O75473), corresponding to amino acid residues F672-T722.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated
(Please refer to the vial label for the specific concentration.)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

leucine rich repeat containing G protein-coupled receptor 5 , FEX , GPR49 , GPR67 , GRP49 , HG38

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-LGR5 antibody used in IHC (Paraffin sections) (IHC-P). GTX04555

GTX04555 IHC-P Image

IHC-P analysis of human normal tissues adjacent to gastric cancer tissue using GTX04555 LGR5 antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

Anti-LGR5 antibody used in IHC (Paraffin sections) (IHC-P). GTX04555

GTX04555 IHC-P Image

IHC-P analysis of human colorectal cancer tissue using GTX04555 LGR5 antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

Anti-LGR5 antibody used in IHC (Paraffin sections) (IHC-P). GTX04555

GTX04555 IHC-P Image

IHC-P analysis of human mammary cancer tissue using GTX04555 LGR5 antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

Anti-LGR5 antibody used in IHC (Paraffin sections) (IHC-P). GTX04555

GTX04555 IHC-P Image

IHC-P analysis of human esophageal cancer tissue using GTX04555 LGR5 antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

Anti-LGR5 antibody used in IHC (Paraffin sections) (IHC-P). GTX04555

GTX04555 IHC-P Image

IHC-P analysis of human esophageal cancer tissue using GTX04555 LGR5 antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

Anti-LGR5 antibody used in Western Blot (WB). GTX04555

GTX04555 WB Image

WB analysis of HEK293 whole cell lysate using GTX04555 LGR5 antibody. The lane on the left was treated with blocking peptide.

Anti-LGR5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04555

GTX04555 ICC/IF Image

ICC/IF analysis of PFA fixed HeLa cells using GTX04555 LGR5 antibody.
Red : Primary antibody
Green : Beta tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

Anti-LGR5 antibody used in IHC (Paraffin sections) (IHC-P). GTX04555

GTX04555 IHC-P Image

IHC-P analysis of human mammary cancer tissue using GTX04555 LGR5 antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

Anti-LGR5 antibody used in IHC (Paraffin sections) (IHC-P). GTX04555

GTX04555 IHC-P Image

IHC-P analysis of human liver cancer tissue using GTX04555 LGR5 antibody.
Antigen retrieval : Heat mediated citrate buffer
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 399