APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:500-1:3000 |
Note :
WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.
|
Not tested in other applications.
Calculated MW
Positive Control
Myc-human LGR5-transfected 293T (un-boiled)
Predict Reactivity
Mouse, Rat, Bovine, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human LGR5. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886046
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
leucine rich repeat containing G protein-coupled receptor 5 , FEX , GPR49 , GPR67 , GRP49 , HG38
Cellular Localization
Cell membrane; Multi-pass membrane protein
Background
Orphan receptor. Stem cell marker of the intestinal epithelium and the hair follicule. Target gene of Wnt signaling.
Database
Research Area
DATA IMAGES
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GTX129597 WB Image
LGR5 antibody detects LGR5 protein by western blot analysis. Non-transfected (-, unboiled) and LGR5-transfected (+, unboiled) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LGR5 antibody (GTX129597) diluted at 1:1000.
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REFERENCE
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REVIEW
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