PRODUCT
Summary
LGR5 antibody recognizes leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5), a receptor protein with a predicted molecular weight of 100 kDa. LGR5, also known as GPR49 or GPR67, is a crucial determinant of the stem cell niche responsible for tissue regeneration in various organs such as the small intestine, colon, and hair follicle. In addition, LGR5 expression is found in cancer stem cells of various tumor types.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:10000 |
| Assay dependent |
| Assay dependent |
|
Note :
WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.
IHC-P
Recommended citrate buffer (pH 6.0) heat-mediated antigen retrieval. The optimal antigen retrieval time is assay dependent. The average time is 2~3 mins. Longer than 5 minutes may destroy the epitope.
|
Not tested in other applications.
Calculated MW
Predict Reactivity
Rat, Bovine, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.56 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the Extracellular domain of human LGR5. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886205
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
leucine rich repeat containing G protein-coupled receptor 5 , FEX , GPR49 , GPR67 , GRP49 , HG38
Cellular Localization
Cell membrane; Multi-pass membrane protein
Background
Orphan receptor. Stem cell marker of the intestinal epithelium and the hair follicule. Target gene of Wnt signaling.
Database
Research Area
DATA IMAGES
|
GTX130204 IHC-P Image
LGR5 antibody detects LGR5 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.LGR5 stained by LGR5 antibody (GTX130204) diluted at 1:25.Antigen Retrieval: Citrate buffer, pH 6.0, 5 min
|
|
|
GTX130204 ICC/IF Image
LGR5 antibody detects LGR5 protein at cell membrane by immunofluorescent analysis.Sample: COLO 205 (high expression) and RMS13 (low expression) cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: LGR5 stained by LGR5 antibody (GTX130204) diluted at 1:200.Blue: Fluoroshield with DAPI (GTX30920).
|
|
|
GTX130204 IHC-P Image
LGR5 antibody detects LGR5 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.LGR5 stained by LGR5 antibody (GTX130204) diluted at 1:25.Antigen Retrieval: Citrate buffer, pH 6.0, 3 min
|
|
|
GTX130204 WB Image
Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with LGR5 antibody (GTX130204) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
|
|
|
GTX130204 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LGR5 antibody (GTX130204) diluted at 1:5000.
|
|
|
GTX130204 WB Image
Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LGR5 antibody (GTX130204) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
REFERENCE
There are currently no references for LGR5 antibody (GTX130204). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for LGR5 antibody (GTX130204). Be the first to share your experience with this product.
