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LGR5 antibody

Anti-LGR5 antibody used in IHC (Paraffin sections) (IHC-P). GTX130204
Anti-LGR5 antibody used in Western Blot (WB). GTX130204
Anti-LGR5 antibody used in Western Blot (WB). GTX130204
Anti-LGR5 antibody used in Western Blot (WB). GTX130204
Anti-LGR5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130204
Anti-LGR5 antibody used in IHC (Paraffin sections) (IHC-P). GTX130204

Cat. No. GTX130204

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)
PRODUCT

Summary

LGR5 antibody recognizes leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5), a receptor protein with a predicted molecular weight of 100 kDa. LGR5, also known as GPR49 or GPR67, is a crucial determinant of the stem cell niche responsible for tissue regeneration in various organs such as the small intestine, colon, and hair follicle. In addition, LGR5 expression is found in cancer stem cells of various tumor types.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent

Note :

WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.

IHC-P
Recommended citrate buffer (pH 6.0) heat-mediated antigen retrieval. The optimal antigen retrieval time is assay dependent. The average time is 2~3 mins. Longer than 5 minutes may destroy the epitope.

Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human LGR5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886205

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

leucine rich repeat containing G protein-coupled receptor 5 , FEX , GPR49 , GPR67 , GRP49 , HG38

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Orphan receptor. Stem cell marker of the intestinal epithelium and the hair follicule. Target gene of Wnt signaling.

Database

Research Area

DATA IMAGES

Anti-LGR5 antibody used in IHC (Paraffin sections) (IHC-P). GTX130204

GTX130204 IHC-P Image

LGR5 antibody detects LGR5 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
LGR5 stained by LGR5 antibody (GTX130204) diluted at 1:25.
Antigen Retrieval: Citrate buffer, pH 6.0, 5 min

Anti-LGR5 antibody used in Western Blot (WB). GTX130204

GTX130204 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with LGR5 antibody (GTX130204) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-LGR5 antibody used in Western Blot (WB). GTX130204

GTX130204 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LGR5 antibody (GTX130204) diluted at 1:5000.

Anti-LGR5 antibody used in Western Blot (WB). GTX130204

GTX130204 WB Image

Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LGR5 antibody (GTX130204) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LGR5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130204

GTX130204 ICC/IF Image

LGR5 antibody detects LGR5 protein at cell membrane by immunofluorescent analysis.
Sample: COLO 205 (high expression) and RMS13 (low expression) cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LGR5 stained by LGR5 antibody (GTX130204) diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-LGR5 antibody used in IHC (Paraffin sections) (IHC-P). GTX130204

GTX130204 IHC-P Image

LGR5 antibody detects LGR5 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
LGR5 stained by LGR5 antibody (GTX130204) diluted at 1:25.
Antigen Retrieval: Citrate buffer, pH 6.0, 3 min

REFERENCE

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169