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LHX2 antibody

Anti-LHX2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX129241
Anti-LHX2 antibody used in Western Blot (WB). GTX129241
Anti-LHX2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX129241
Anti-LHX2 antibody used in Western Blot (WB). GTX129241
Anti-LHX2 antibody used in Western Blot (WB). GTX129241

Cat. No. GTX129241

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Fr, IHC-Wm

Reactivity

 Human, Mouse, Rat, Japanese Medaka
Package
100 μl ($399),
25 μl ($169)
See all LHX2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
IHC-Fr 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

Neuro2A , GL261 , C8D30 , NIH-3T3 , EBCL-1 , FBCL-1 , Raw264.7 , C2C12 , mouse brain , mouse eye

Predict Reactivity

Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human LHX2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2783558

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

LIM homeobox 2 , LH2 , hLhx2

Cellular Localization

Nucleus

Background

This gene encodes a protein belonging to a large protein family, members of which carry the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator. The protein can recapitulate or rescue phenotypes in Drosophila caused by a related protein, suggesting conservation of function during evolution. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LHX2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX129241

GTX129241 IHC-Fr Image

LHX2 antibody detects LHX2 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: LHX2 protein stained by LHX2 antibody (GTX129241) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-LHX2 antibody used in Western Blot (WB). GTX129241

GTX129241 WB Image

LHX2 antibody detects LHX2 protein by western blot analysis.
A. Neuro2A whole cell lysate/extract
B. GL261 whole cell lysate/extract
C. C8D30 whole cell lysate/extract
D. NIH-3T3 whole cell lysate/extract
E. BCL-1 whole cell lysate/extract
F. BCL-1 whole cell lysate/extract
G. Raw264.7 whole cell lysate/extract
H. C2C12 whole cell lysate/extract
10% SDS-PAGE
LHX2 antibody (GTX129241) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LHX2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX129241

GTX129241 IHC-Fr Image

LHX2 antibody detects LHX2 protein by immunohistochemical analysis.
Sample: Frozen-sectioned rat E13.5 brain.
Green: LHX2 stained by LHX2 antibody (GTX129241) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-LHX2 antibody used in Western Blot (WB). GTX129241

GTX129241 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LHX2 antibody (GTX129241) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LHX2 antibody used in Western Blot (WB). GTX129241

GTX129241 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with LHX2 antibody (GTX129241) diluted at 1:10000 (left panel) and 1:5000 (right panel). The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Jung S et al. Nat Commun 2024; 15 (1) : 158 Role of UPF1-LIN28A interaction during early differentiation of pluripotent stem cells.
Zilova L et al. Elife 2021; 10 Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development.
Application : IHC-Wm
Reactivity : Japanese Medaka
Kano M et al. Endocrinology 2019; (Epub) Tanycyte-like cells derived from mouse embryonic stem culture show hypothalamic neural stem/progenitor cell functions.
Biehs B et al. Nature 2018; 562 (7727) : 429-433. A cell identity switch allows residual BCC to survive Hedgehog pathway inhibition.
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169