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LHX2 antibody

Anti-LHX2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX129241
Anti-LHX2 antibody used in Western Blot (WB). GTX129241
Anti-LHX2 antibody used in Western Blot (WB). GTX129241
Anti-LHX2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX129241
Anti-LHX2 antibody used in Western Blot (WB). GTX129241

Cat No. GTX129241

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Neuro 2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , mouse brain , mouse eye

Predict Reactivity

Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human LHX2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

LIM homeobox 2 , LH2 , hLhx2

Cellular Localization

Nucleus

Background

This gene encodes a protein belonging to a large protein family, members of which carry the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator. The protein can recapitulate or rescue phenotypes in Drosophila caused by a related protein, suggesting conservation of function during evolution. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LHX2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX129241

GTX129241 IHC-Fr Image

LHX2 antibody detects LHX2 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: LHX2 protein stained by LHX2 antibody (GTX129241) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-LHX2 antibody used in Western Blot (WB). GTX129241

GTX129241 WB Image

LHX2 antibody detects LHX2 protein by western blot analysis.
A. Neuro2A whole cell lysate/extract
B. GL261 whole cell lysate/extract
C. C8D30 whole cell lysate/extract
D. NIH-3T3 whole cell lysate/extract
E. BCL-1 whole cell lysate/extract
F. BCL-1 whole cell lysate/extract
G. Raw264.7 whole cell lysate/extract
H. C2C12 whole cell lysate/extract
10% SDS-PAGE
LHX2 antibody (GTX129241) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LHX2 antibody used in Western Blot (WB). GTX129241

GTX129241 WB Image

LHX2 antibody detects LHX2 protein by western blot analysis.
A 293T whole cell lysate/extract
B A431 whole cell lysate/extract
C HeLa
D HepG2 whole cell lysate/extract
10% SDS-PAGE
LHX2 antibody (GTX129241) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LHX2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX129241

GTX129241 IHC-Fr Image

LHX2 antibody detects LHX2 protein by immunohistochemical analysis.
Sample: Frozen-sectioned rat E13.5 brain.
Green: LHX2 stained by LHX2 antibody (GTX129241) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-LHX2 antibody used in Western Blot (WB). GTX129241

GTX129241 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with LHX2 antibody (GTX129241) diluted at 1:10000 (left panel) and 1:5000 (right panel). The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169