ICC: Use at a dilution of 1/100 - 1/250. IF: Use at an assay dependent dilution. Use a secondary antibody conjugated to biotin and then streptavidin-conjugated to fluorochrome. IHC-P: Use at a dilution of 1/125 - 1/500. IHC-Fr: 1:1500-1:3000. For whole mounts recommended fixative is MEMFA using a dilution of 1/2000 - 1/4000. IP: Use at a dilution of 1/100. WB: Use at a dilution of 1/1250 - 1/2500. Predicted molecular weight: 44 kDa. Optimal dilutions/concentrations should be determined by the end user.
GTX14555 recognizes LHX3 and does not appear to crossreact with gsh4 on tissue sections.
Preservative: 0.1% Sodium Azide; Constituents: PBS. pH 7.2
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
C-terminal portion of mouse LHX3 protein.
Protein A purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Lim Homeobox Protein 3,Lim3,P-Lim,Mlim3,Mlim-3,Lhx3
The pituitary gland releases hormones that regulate growth, lactation, homeostasis, the stress response, reproductive development and fitness as well as water metabolism. LHX3 is one of several transcription factors that have been implicated in pituitary development. This particular transcription factor is critical for motor neuron specification and the development of the anterior and intermediate lobes of the pituitary gland in mammals. The sequence of the human LHX3 gene has been identified and characterized by IU researchers.