GeneTex
United States (US)

LHX3 antibody

Cat No. GTX14555

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IP
Reactivity Human, Mouse, Rat, Frog, Fish
APPLICATION

Application Note

ICC: Use at a dilution of 1/100 - 1/250. IF: Use at an assay dependent dilution. Use a secondary antibody conjugated to biotin and then streptavidin-conjugated to fluorochrome. IHC-P: Use at a dilution of 1/125 - 1/500. IHC-Fr: 1:1500-1:3000. For whole mounts recommended fixative is MEMFA using a dilution of 1/2000 - 1/4000. IP: Use at a dilution of 1/100. WB: Use at a dilution of 1/1250 - 1/2500. Predicted molecular weight: 44 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

44 kDa. ( Note )

Specificity/Sensitivity

GTX14555 recognizes LHX3 and does not appear to crossreact with gsh4 on tissue sections.
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.1% Sodium Azide; Constituents: PBS. pH 7.2

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

C-terminal portion of mouse LHX3 protein.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Lim Homeobox Protein 3,Lim3,P-Lim,Mlim3,Mlim-3,Lhx3

Cellular Localization

Nuclear

Background

The pituitary gland releases hormones that regulate growth, lactation, homeostasis, the stress response, reproductive development and fitness as well as water metabolism. LHX3 is one of several transcription factors that have been implicated in pituitary development. This particular transcription factor is critical for motor neuron specification and the development of the anterior and intermediate lobes of the pituitary gland in mammals. The sequence of the human LHX3 gene has been identified and characterized by IU researchers.

Database

Package List Price ($)
$ 329