GeneTex
United States (US)

LIF antibody

GTX101021_42872_20171006_WB_competitor_watermark_19011506_590.jpg
GTX101021_42872_20170608_WB_19011506_330.jpg
GTX101021_42872_20170608_WB_2_19011506_856.jpg

Cat No. GTX101021

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , HUVEC

Predict Reactivity

Rabbit, Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.07 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LIF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Lif, Interleukin 6 Family Cytokine,Cdf,Dia,Hilda,Mlpli,Lif

Cellular Localization

Secreted

Background

The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101021_42872_20171006_WB_competitor_watermark_19011506_590.jpg

GTX101021 WB Image

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membranes were blotted with LIF antibody [N1C3] (GTX101021) diluted at 1:500 and competitor's antibody (sc-1336) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX101021_42872_20170608_WB_19011506_330.jpg

GTX101021 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with LIF antibody (GTX101021) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101021_42872_20170608_WB_2_19011506_856.jpg

GTX101021 WB Image

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with LIF antibody (GTX101021) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169