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LIF antibody

Anti-LIF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31431
Anti-LIF antibody used in Western Blot (WB). GTX31431

Cat. No. GTX31431

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all LIF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
ICC/IF 20 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

EL4 Cell Lysate(GTX27183) , 3T3/BALB Cell Slide

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

LIF antibody was raised against a 16 amino acid synthetic peptide near the center of human LIF.The immunogen is located within amino acids 50 - 100 of LIF.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

LIF interleukin 6 family cytokine , CDF , DIA , HILDA , MLPLI

Cellular Localization

Secreted

Background

The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]

Database

Research Area

DATA IMAGES

Anti-LIF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31431

GTX31431 ICC/IF Image

ICC/IF analysis of 3T3 cells using GTX31431 LIF antibody.
Working concentration : 20 μg/ml

Anti-LIF antibody used in Western Blot (WB). GTX31431

GTX31431 WB Image

WB analysis of 3T3 cell lysate using GTX31431 LIF antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

REFERENCE

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399