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LILRB1 antibody [N1C1]

Anti-LILRB1 antibody [N1C1] used in Western Blot (WB). GTX112718
Anti-LILRB1 antibody [N1C1] used in Western Blot (WB). GTX112718
Anti-LILRB1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112718

Cat. No. GTX112718

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($419),
25 μl ($169)
See all LILRB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human LILRB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10722986

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

leukocyte immunoglobulin like receptor B1 , CD85J , ILT-2 , ILT2 , LIR-1 , LIR1 , MIR-7 , MIR7 , PIR-B , PIRB

Background

This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LILRB1 antibody [N1C1] used in Western Blot (WB). GTX112718

GTX112718 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with LILRB1 antibody [N1C1] (GTX112718) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LILRB1 antibody [N1C1] used in Western Blot (WB). GTX112718

GTX112718 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
7.5% SDS PAGE
GTX112718 diluted at 1:10000

Anti-LILRB1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112718

GTX112718 ICC/IF Image

LILRB1 antibody [N1C1] detects LILRB1 protein by immunofluorescent analysis.
Sample: NK-92 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LILRB1 stained by LILRB1 antibody [N1C1] (GTX112718) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169