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LIM kinase 2 (phospho Thr505) antibody

Anti-LIM kinase 2 (phospho Thr505) antibody used in Western Blot (WB). GTX50188
Anti-LIM kinase 2 (phospho Thr505) antibody used in IHC (Paraffin sections) (IHC-P). GTX50188
Anti-LIM kinase 2 (phospho Thr505) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50188

Cat No. GTX50188

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 505 (R-Y-T(p)-V-V) derived from human LIM kinase 2.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

RRID

AB_11162973

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

LIM domain kinase 2

Cellular Localization

Nucleus

Background

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-LIM kinase 2 (phospho Thr505) antibody used in Western Blot (WB). GTX50188

GTX50188 WB Image

WB analysis of extracts from HeLa cells untreated or treated with PMA using GTX50188 LIM kinase 2 (phospho Thr505) antibody.

Anti-LIM kinase 2 (phospho Thr505) antibody used in IHC (Paraffin sections) (IHC-P). GTX50188

GTX50188 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50188 LIM kinase 2 (phospho Thr505) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-LIM kinase 2 (phospho Thr505) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50188

GTX50188 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50188 LIM kinase 2 (phospho Thr505) antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339