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LIM kinase 2 (phospho Thr505) antibody

Anti-LIM kinase 2 (phospho Thr505) antibody used in Western Blot (WB). GTX79022
Anti-LIM kinase 2 (phospho Thr505) antibody used in IHC (Paraffin sections) (IHC-P). GTX79022

Cat No. GTX79022

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human LIMK2 around the phosphorylation site of threonine 505 (R-Y-Tp-V-V).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

LIM domain kinase 2,LIMK2,LIM Kinase 2

Cellular Localization

Cytoplasm,Nucleus

Background

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-LIM kinase 2 (phospho Thr505) antibody used in Western Blot (WB). GTX79022

GTX79022 WB Image

WB analysis of NIH/3T3 cells treated or untreated with UV (60min) lysates using GTX79022 LIM kinase 2 (phospho Thr505) antibody.

Anti-LIM kinase 2 (phospho Thr505) antibody used in IHC (Paraffin sections) (IHC-P). GTX79022

GTX79022 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX79022 LIM kinase 2 (phospho Thr505) antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 339