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LIM1 antibody [HL2774]

Recombinant antibody Orthogonal
Anti-LIM1 antibody [HL2774] used in IHC (Paraffin sections) (IHC-P). GTX639639
Anti-LIM1 antibody [HL2774] used in IHC (Paraffin sections) (IHC-P). GTX639639
Anti-LIM1 antibody [HL2774] used in Western Blot (WB). GTX639639

Cat. No. GTX639639

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2774

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Mouse
Package
100 μl ($399),
25 μl ($169)
See all LIM1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human LIM1.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

LIM homeobox 1 , LIM-1 , LIM1

Cellular Localization

Nucleus

Background

This gene encodes a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein is a transcription factor important for the development of the renal and urogenital systems. This gene is a candidate for Mayer-Rokitansky-Kuster-Hauser syndrome, a disorder characterized by anomalies in the female genital tract. [provided by RefSeq, Dec 2010]

Database

Research Area

DATA IMAGES

Anti-LIM1 antibody [HL2774] used in IHC (Paraffin sections) (IHC-P). GTX639639

GTX639639 IHC-P Image

LIM1 antibody detects LIM1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
LIM1 stained by LIM1 antibody (GTX639639) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LIM1 antibody [HL2774] used in IHC (Paraffin sections) (IHC-P). GTX639639

GTX639639 IHC-P Image

LIM1 antibody detects LIM1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: LIM1 stained by LIM1 antibody (GTX639639) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Hoechst 33342 staining.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LIM1 antibody [HL2774] used in Western Blot (WB). GTX639639

GTX639639 WB Image

Orthogonal

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LIM1 antibody [HL2774] (GTX639639) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations