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LIM1 antibody

Anti-LIM1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX129215
Anti-LIM1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX129215
Anti-LIM1 antibody used in IHC (Paraffin sections) (IHC-P). GTX129215
Anti-LIM1 antibody used in IHC (Paraffin sections) (IHC-P). GTX129215
Anti-LIM1 antibody used in Western Blot (WB). GTX129215
Anti-LIM1 antibody used in Western Blot (WB). GTX129215
Anti-LIM1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129215
Anti-LIM1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129215

Cat No. GTX129215

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Bovine, Chicken, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.05 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LIM1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

LIM homeobox 1 , LIM-1 , LIM1

Cellular Localization

Nucleus

Background

This gene encodes a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator and be involved in control of differentiation and development of neural and lymphoid cells. A similar protein in mice is an essential regulator of the vertebrate head organizer. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LIM1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX129215

GTX129215 IHC-Fr Image

LIM1 antibody detects LIM1 protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: LIM1 protein stained by LIM1 antibody (GTX129215) diluted at 1:250.
Red: Protein kinase C alpha staining.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-LIM1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX129215

GTX129215 IHC-Fr Image

LIM1 antibody detects LIM1 protein expression at nucleus by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: LIM1 protein stained by LIM1 antibody (GTX129215) diluted at 1:250.
Red: SOX2, stained by SOX2 antibody [GT1876] (GTX627404) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-LIM1 antibody used in IHC (Paraffin sections) (IHC-P). GTX129215

GTX129215 IHC-P Image

LIM1 antibody detects LIM1 protein at nucleus on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
LIM1 antibody (GTX129215) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-LIM1 antibody used in IHC (Paraffin sections) (IHC-P). GTX129215

GTX129215 IHC-P Image

LIM1 antibody detects LIM1 protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
LIM1 antibody (GTX129215) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LIM1 antibody used in Western Blot (WB). GTX129215

GTX129215 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with LIM1 antibody (GTX129215) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LIM1 antibody used in Western Blot (WB). GTX129215

GTX129215 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with LIM1 antibody (GTX129215) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LIM1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129215

GTX129215 ICC/IF Image

LIM1 antibody detects LIM1 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LIM1 stained by LIM1 antibody (GTX129215) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-LIM1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129215

GTX129215 ICC/IF Image

LIM1 antibody detects LIM1 protein at nucleus by immunofluorescent analysis.
Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LIM1 stained by LIM1 antibody (GTX129215) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 μm.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169