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LIMCH1 antibody [C2C3], C-term

Anti-LIMCH1 antibody [C2C3], C-term used in Western Blot (WB). GTX108649
Anti-LIMCH1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108649
Anti-LIMCH1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108649
Anti-LIMCH1 antibody [C2C3], C-term used in Western Blot (WB). GTX108649
Anti-LIMCH1 antibody [C2C3], C-term used in Western Blot (WB). GTX108649

Cat No. GTX108649

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

122 kDa. ( Note )

Positive Control

H1299 , HeLaS3 , mouse brain

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human LIMCH1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950813

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

LIM and calponin homology domains 1 , LIMCH1A , LMO7B

Database

Research Area

DATA IMAGES

Anti-LIMCH1 antibody [C2C3], C-term used in Western Blot (WB). GTX108649

GTX108649 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LIMCH1 antibody [C2C3], C-term (GTX108649) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-LIMCH1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108649

GTX108649 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using LIMCH1(GTX108649) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LIMCH1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108649

GTX108649 ICC/IF Image

LIMCH1 antibody [C2C3], C-term detects LIMCH1 protein at stress fiber by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: LIMCH1 stained by LIMCH1 antibody [C2C3], C-term (GTX108649) diluted at 1:500.

Anti-LIMCH1 antibody [C2C3], C-term used in Western Blot (WB). GTX108649

GTX108649 WB Image

Wild-type (WT) and LIMCH1 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LIMCH1 antibody [C2C3], C-term (GTX108649) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LIMCH1 antibody [C2C3], C-term used in Western Blot (WB). GTX108649

GTX108649 WB Image

(30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LIMCH1 antibody [C2C3], C-term (GTX108649) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for LIMCH1 antibody [C2C3], C-term (GTX108649). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for LIMCH1 antibody [C2C3], C-term (GTX108649). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169