GeneTex
United States (US)

LIMCH1 antibody [C2C3], C-term

GTX108649_40002_20181109_WB_KO_watermark_19011519_745.jpg
GTX108649_40002_20181109_WB_M_brain_19011519_227.jpg
GTX108649_40002_WB_M_brain_19011507_623.jpg
GTX108649_40002_IHC_19011507_405.jpg
GTX108649_WB_19011507_250.jpg

Cat No. GTX108649

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

122 kDa. ( Note )

Positive Control

H1299 , HeLaS3 , mouse brain

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human LIMCH1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DKFZp434I0312 antibody, DKFZp686A01247 antibody, DKFZp686B2470 antibody, DKFZp686G18243 antibody, DKFZp686G2094 antibody, DKFZp781C1754 antibody, DKFZp781I1455 antibody, LIMCH1A antibody, LMO7B antibody, MGC72127 antibody, LIMCH1 antibody, LIM and calponin homology domains-containing protein 1 antibody, LIM and calponin homology domains 1 antibody

Database

DATA IMAGES
GTX108649_40002_20181109_WB_KO_watermark_19011519_745.jpg

GTX108649 WB Image

Wild-type (WT) and LIMCH1 knockout (KO) HeLa cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with LIMCH1 antibody [C2C3], C-term (GTX108649) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108649_40002_20181109_WB_M_brain_19011519_227.jpg

GTX108649 WB Image

(30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with LIMCH1 antibody [C2C3], C-term (GTX108649) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108649_40002_WB_M_brain_19011507_623.jpg

GTX108649 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
GTX108649 diluted at 1:2000

GTX108649_40002_IHC_19011507_405.jpg

GTX108649 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using LIMCH1(GTX108649) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX108649_WB_19011507_250.jpg

GTX108649 WB Image

Sample (30μg whole cell lysate)
A:H1299
7.5% SDS PAGE
GTX108649 diluted at 1:3000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169