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LIMD1 antibody [C2C3], C-term

Anti-LIMD1 antibody [C2C3], C-term used in Western Blot (WB). GTX106685
Anti-LIMD1 antibody [C2C3], C-term used in Western Blot (WB). GTX106685
Anti-LIMD1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106685
Anti-LIMD1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106685
Anti-LIMD1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106685

Cat. No. GTX106685

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all LIMD1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

Mouse brain , 293T

Predict Reactivity

Rat, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human LIMD1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950814

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

LIM domains containing 1

Background

Transcriptional regulator that inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. May act as a tumor suppressor by inhibiting cell proliferation.

Database

Research Area

DATA IMAGES

Anti-LIMD1 antibody [C2C3], C-term used in Western Blot (WB). GTX106685

GTX106685 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX106685 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LIMD1 antibody [C2C3], C-term used in Western Blot (WB). GTX106685

GTX106685 WB Image

Sample(30 μg whole cell lysate)
A:293T
12% SDS PAGE
GTX106685 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LIMD1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106685

GTX106685 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using LIMD1(GTX106685) antibody at 1:300 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-LIMD1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106685

GTX106685 IHC-P Image

LIMD1 antibody [C2C3], C-term detects LIMD1 protein at nucleus on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
LIMD1 antibody [C2C3], C-term (GTX106685) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-LIMD1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106685

GTX106685 ICC/IF Image

LIMD1 antibody [C2C3], C-term detects LIMD1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LIMD1 protein stained by LIMD1 antibody [C2C3], C-term (GTX106685) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169