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LIMD1 antibody [C2C3], C-term

Anti-LIMD1 antibody [C2C3], C-term used in Western Blot (WB). GTX106685
Anti-LIMD1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106685
Anti-LIMD1 antibody [C2C3], C-term used in Western Blot (WB). GTX106685
Anti-LIMD1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106685
Anti-LIMD1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106685

Cat No. GTX106685

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

Positive Control

293T , mouse brain

Predict Reactivity

Rat, Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human LIMD1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950814

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

LIM domains containing 1

Background

Transcriptional regulator that inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. May act as a tumor suppressor by inhibiting cell proliferation.

Database

Research Area

DATA IMAGES

Anti-LIMD1 antibody [C2C3], C-term used in Western Blot (WB). GTX106685

GTX106685 WB Image

Sample(30 μg whole cell lysate)
A:293T
12% SDS PAGE
GTX106685 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LIMD1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106685

GTX106685 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using LIMD1(GTX106685) antibody at 1:300 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-LIMD1 antibody [C2C3], C-term used in Western Blot (WB). GTX106685

GTX106685 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX106685 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LIMD1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106685

GTX106685 IHC-P Image

LIMD1 antibody [C2C3], C-term detects LIMD1 protein at nucleus on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
LIMD1 antibody [C2C3], C-term (GTX106685) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-LIMD1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106685

GTX106685 ICC/IF Image

LIMD1 antibody [C2C3], C-term detects LIMD1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LIMD1 protein stained by LIMD1 antibody [C2C3], C-term (GTX106685) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

There are currently no references for LIMD1 antibody [C2C3], C-term (GTX106685). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for LIMD1 antibody [C2C3], C-term (GTX106685). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf