GeneTex
United States (US)

LIME antibody [LIME-10]

LIME-antibody-LIME-10__GTX14987-1_18121411_110.jpg

Cat No. GTX14987

Host Mouse
Clonality Monoclonal
Clone Name LIME-10
Isotype IgG2a
Application WB
Reactivity Human
APPLICATION

Application Note

WB: Use at an assay dependent dilution. Predicted molecular weight: 32.3 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

31 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline pH 7.4 containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

COOH-terminal peptide comprising residues 281-296 of the human LIME conjugated to keyhole limpet hemocyanin.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Lck Interacting Transmembrane Adaptor 1,Lime,Dj583P15.4,Lime1

Background

LIME (Lck-interacting molecule) is a new raft-associated adaptor protein involved in regulation of T cell activation by coreceptors. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation.

Database

Research Area

DATA IMAGES
LIME-antibody-LIME-10__GTX14987-1_18121411_110.jpg

GTX14987 WB Image

Western Blot analysis (reducing conditions) of human LIME using anti-human LIME (GTX14987).
Lane 1: J77 cell line transfected with LIME
Lane 2: non-transfected J77 cell line (cells are essentially devoid of endogenous LIME expression)

Package List Price ($)
$ 319