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LIMK1 antibody

Anti-LIMK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02581
Anti-LIMK1 antibody used in Western Blot (WB). GTX02581

Cat No. GTX02581

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IP, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500
IHC-P 1:50-1:150
IP 1:200
ELISA 1:10,000
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tris/Glycine pH7.4-7.9, 30% Glycerol, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide taken within amino acid region 550-647 on human LIM Kinase 1 protein.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

LIM domain kinase 1 , LIMK , LIMK-1

Cellular Localization

Cytoplasm , Nucleus

Background

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-LIMK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX02581

GTX02581 IHC-P Image

IHC-P analysis of mouse skeletal muscle using GTX02581 LIMK1 antibody.
Dilution : 1:50

Anti-LIMK1 antibody used in Western Blot (WB). GTX02581

GTX02581 WB Image

WB analysis of THP-1 cell lysate using GTX02581 LIMK1 antibody.
Dilution : 1:500

REFERENCE

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REVIEW

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Package List Price ($)
$ 399