WB analysis of mouse brain tissue lysate using GTX10561 LIMK1 antibody.
Loading : 18 μg
Dilution : 1:500

Western blot using GeneTex Affinity Purified anti-LIM Kinase antibody (GTX10561) shows detection of a 73 kDa band corresponding to LIM kinase in lysates from mouse brain. Approximately 18 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-LIM kinase antibody. The doublet band at ~75 kDa may represent phosphorylated and non-phosphorylated forms of the protein. The identity of the strong lower molecular weight band at approximately 20 kDa is unknown. Signal was detected using standard techniques.