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LIMK1 (phospho Thr508) + LIMK2 (phospho Thr505) antibody

Anti-LIMK1 (phospho Thr508) + LIMK2 (phospho Thr505) antibody used in Western Blot (WB). GTX50187
Anti-LIMK1 (phospho Thr508) + LIMK2 (phospho Thr505) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50187
Anti-LIMK1 (phospho Thr508) + LIMK2 (phospho Thr505) antibody used in Western Blot (WB). GTX50187
Anti-LIMK1 (phospho Thr508) + LIMK2 (phospho Thr505) antibody used in IHC (Paraffin sections) (IHC-P). GTX50187

Cat No. GTX50187

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 508 (R-Y-T(p)-V-V) derived from human LIMK1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

LIM domain kinase 1 , LIMK , LIMK-1

Cellular Localization

Cytoplasm,Nucleus

Background

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-LIMK1 (phospho Thr508) + LIMK2 (phospho Thr505) antibody used in Western Blot (WB). GTX50187

GTX50187 WB Image

WB analysis of extracts from various sample using GTX50187 LIMK1 (phospho Thr508)  + LIMK2 (phospho Thr505) antibody.
Lane 1 : HeLa  treated with PMA
Lane 2 : Mouse brain tissue
Lane 3 : Rat brain tissue
Lane 4 : N-peptide blocked HeLa treated with PMA
Lane 5 : N-peptide blocked Mouse brain tissue
Lane 6 : N-peptide blocked Rat brain tissue
Lane 7 : P-peptide blocked HeLa  treated with PMA
Lane 8 : P-peptide blocked Mouse brain tissue
Lane 9 : P-peptide blocked Rat brain tissue
Loading : 40 μg per lane

Anti-LIMK1 (phospho Thr508) + LIMK2 (phospho Thr505) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50187

GTX50187 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50187 LIMK1 (phospho Thr508)  + LIMK2 (phospho Thr505) antibody.

Anti-LIMK1 (phospho Thr508) + LIMK2 (phospho Thr505) antibody used in Western Blot (WB). GTX50187

GTX50187 WB Image

WB analysis of extracts from UV-treated C2C12, PMA-treated HeLa, and anisomycin-treated 293 cells using GTX50187 LIMK1 (phospho Thr508)  + LIMK2 (phospho Thr505) antibody.

Anti-LIMK1 (phospho Thr508) + LIMK2 (phospho Thr505) antibody used in IHC (Paraffin sections) (IHC-P). GTX50187

GTX50187 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50187 LIMK1 (phospho Thr508)  + LIMK2 (phospho Thr505) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379