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LIMK1 (phospho Thr508) + LIMK2 (phospho Thr505) antibody

Orthogonal
Anti-LIMK1 (phospho Thr508) + LIMK2 (phospho Thr505) antibody used in IHC (Paraffin sections) (IHC-P). GTX50187
Anti-LIMK1 (phospho Thr508) + LIMK2 (phospho Thr505) antibody used in Western Blot (WB). GTX50187
Anti-LIMK1 (phospho Thr508) + LIMK2 (phospho Thr505) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50187
Anti-LIMK1 (phospho Thr508) + LIMK2 (phospho Thr505) antibody used in Western Blot (WB). GTX50187

Cat. No. GTX50187

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all LIMK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 508 (R-Y-T(p)-V-V) derived from human LIMK1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

LIM domain kinase 1 , LIMK , LIMK-1

Cellular Localization

Cytoplasm,Nucleus

Background

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-LIMK1 (phospho Thr508) + LIMK2 (phospho Thr505) antibody used in IHC (Paraffin sections) (IHC-P). GTX50187

GTX50187 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50187 LIMK1 (phospho Thr508)  + LIMK2 (phospho Thr505) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-LIMK1 (phospho Thr508) + LIMK2 (phospho Thr505) antibody used in Western Blot (WB). GTX50187

GTX50187 WB Image

WB analysis of extracts from UV-treated C2C12, PMA-treated HeLa, and anisomycin-treated 293 cells using GTX50187 LIMK1 (phospho Thr508)  + LIMK2 (phospho Thr505) antibody.

Anti-LIMK1 (phospho Thr508) + LIMK2 (phospho Thr505) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50187

GTX50187 ICC/IF Image

Orthogonal

ICC/IF analysis of methanol-fixed HeLa cells using GTX50187 LIMK1 (phospho Thr508)  + LIMK2 (phospho Thr505) antibody.

Anti-LIMK1 (phospho Thr508) + LIMK2 (phospho Thr505) antibody used in Western Blot (WB). GTX50187

GTX50187 WB Image

WB analysis of extracts from various sample using GTX50187 LIMK1 (phospho Thr508)  + LIMK2 (phospho Thr505) antibody.
Lane 1 : HeLa  treated with PMA
Lane 2 : Mouse brain tissue
Lane 3 : Rat brain tissue
Lane 4 : N-peptide blocked HeLa treated with PMA
Lane 5 : N-peptide blocked Mouse brain tissue
Lane 6 : N-peptide blocked Rat brain tissue
Lane 7 : P-peptide blocked HeLa  treated with PMA
Lane 8 : P-peptide blocked Mouse brain tissue
Lane 9 : P-peptide blocked Rat brain tissue
Loading : 40 μg per lane

REFERENCE

Application Reference

Shao Y et al. Genes Brain Behav 2023; 22 (6) : e12863 Impaired erythropoietin-producing hepatocellular B receptors signaling in the prefrontal cortex and hippocampus following maternal immune activation in male rats.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399