GeneTex
United States (US)

LIMP II antibody, Internal

GTX88440_961_IHC-P_18121411_732.jpg
GTX88440_2322_WB_18121411_366.jpg

Cat No. GTX88440

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.3μg/ml
IHC-P 3-5μg/ml

Note :

Human Cerebral Cortex shows lysosomal staining.
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88440-PEP).

Specificity/Sensitivity

This antibody is expected to recognize reported isoform 1 (NP_005497.1) only.

Predict Reactivity

Mouse(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Concentration

0.50mg/ml

Antigen Species

Human

Immunogen

Peptide with sequence C-NKANIQFGDNGTTIS, from the internal region of the protein sequence according to NP_005497.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

LIMPII Antibody , SR BII Antibody , EPM4 Antibody , AMRF Antibody , HLGP85 Antibody , LGP85 Antibody , SCARB2 Antibody , LIMP2 Antibody , CD36L2 Antibody

Background

The protein encoded by this gene is a type III glycoprotein that is located primarily in limiting membranes of lysosomes and endosomes. Earlier studies in mice and rat suggested that this protein may participate in membrane transportation and the reorganization of endosomal/lysosomal compartment. The protein deficiency in mice was reported to impair cell membrane transport processes and cause pelvic junction obstruction, deafness, and peripheral neuropathy. Further studies in human showed that this protein is a ubiquitously expressed protein and that it is involved in the pathogenesis of HFMD (hand, foot, and mouth disease) caused by enterovirus-71 and possibly by coxsackievirus A16. Mutations in this gene caused an autosomal recessive progressive myoclonic epilepsy-4 (EPM4), also known as action myoclonus-renal failure syndrome (AMRF). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]
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Database

Research Area

DATA IMAGES
GTX88440_961_IHC-P_18121411_732.jpg

GTX88440 IHC-P Image

IHC-P analysis of human cerebral cortex using GTX88440 LIMP II antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8µg/ml

GTX88440_2322_WB_18121411_366.jpg

GTX88440 WB Image

WB analysis of human cerebral cortex lysate using GTX88440 LIMP II antibody, Internal.
Dilution : 0.1µg/ml
Loading : 35µg protein in RIPA buffer