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LIMP II antibody

Orthogonal
Anti-LIMP II antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30676
Anti-LIMP II antibody used in Western Blot (WB). GTX30676
Anti-LIMP II antibody used in IHC (Paraffin sections) (IHC-P). GTX30676

Cat. No. GTX30676

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat, Dog, Primate
Package
100 μl ($449)
See all LIMP II products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:50 - 1:250
IHC-P Assay dependent
IP 1:100
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Mouse

Immunogen

A C-terminal synthetic peptide made to the mouse LIMPII/lgp85 protein sequence. [UniProt# O35114]

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_625948

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

scavenger receptor class B, member 2 , 9330185J12Rik , Cd36l2 , LGP85 , LIMP II , LIMP-2 , MLGP85

Cellular Localization

Lysosome membrane

Background

May act as a lysosomal receptor.

Database

Research Area

DATA IMAGES

Anti-LIMP II antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30676

GTX30676 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30676 LIMP II antibody.
Green : primary antibody
Blue : Hoechst 33258

Anti-LIMP II antibody used in Western Blot (WB). GTX30676

GTX30676 WB Image

Orthogonal

WB analysis of multiple samples using GTX30676 LIMP II antibody.
Lane 1: Rat cardiac fibroblasts (RCF)
Lane 2 : 3T3 cells
Lane 3 : dog lung lysate
Lane 4 : RCF treated with angiotensin II
Lane 5 : RCF treated with H2O2
Lane 6 : HEPG2 human liver lysate

Anti-LIMP II antibody used in IHC (Paraffin sections) (IHC-P). GTX30676

GTX30676 IHC-P Image

IHC-P analysis of rat infarctzone tissue using GTX30676 LIMP II antibody.

REFERENCE

Application Reference

Phyu WK et al. Sci Rep 2017; 7 : 45069 Squamous epitheliotropism of Enterovirus A71 in human epidermis and oral mucosa.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 449