APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 μg/mL |
| 2.5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Mouse Liver Tissue Lysate(GTX27935)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
LIMP2 antibody was raised against a 16 amino acid synthetic peptide from near the center of human LIMP2.The immunogen is located within amino acids 70 - 120 of LIMP2.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
scavenger receptor class B member 2 , AMRF , CD36L2 , EPM4 , HLGP85 , LGP85 , LIMP-2 , LIMPII , SR-BII
Cellular Localization
Lysosome membrane
Background
The lysosomal integral membrane protein 2 (LIMP2) is a heavily glycosylated type III transmembrane protein, the majority of which exists in the lumen of the lysosome and a cytoplasmic domain of approximately 20 amino acids. A deficiency of LIMP2 in mice causes uretic pelvic junction obstruction, deafness, and peripheral neuropathy associated with impaired vesicular trafficking and distribution of apically expressed proteins. More recently, LIMP2 was shown to act as a receptor to bind b-glucocerebrosidase, the enzyme defective in Gaucher disease, a lysosomal storage disorder. LIMP2-deficient mice showed missorted as well as secreted b-glucocerebrosidase, suggesting that LIMP2 also functions as the mannose-6-phosphate-independent trafficking receptor. Despite its predicted molecular weight, LIMP2 runs at approximately 80 – 85 kDa in SDS-PAGE.
Database
Research Area
DATA IMAGES
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GTX85033 IHC-P Image
IHC-P analysis of human skeletal muscle tissue using GTX85033 LIMP II antibody.
Working concentration : 2.5 μg/ml
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GTX85033 WB Image
WB analysis of mouse liver tissue lysate in (A) the absence and (B) presence of blocking peptide using GTX85033 LIMP II antibody.
Working concentration : 1 μg/ml
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GTX85033 IHC-P Image
IHC-P analysis of human skeletal muscle tissue using GTX85033 LIMP II antibody.
Working concentration : 20 μg/ml
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REFERENCE
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REVIEW
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