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LIN7B antibody [1C9]

Anti-LIN7B antibody [1C9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84216
Anti-LIN7B antibody [1C9] used in Flow cytometry (FACS). GTX84216
Anti-LIN7B antibody [1C9] used in IHC (Paraffin sections) (IHC-P). GTX84216
Anti-LIN7B antibody [1C9] used in Western Blot (WB). GTX84216
Anti-LIN7B antibody [1C9] used in IHC (Paraffin sections) (IHC-P). GTX84216
Anti-LIN7B antibody [1C9] used in IHC (Paraffin sections) (IHC-P). GTX84216

Cat. No. GTX84216

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1C9

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μl ($519)
See all LIN7B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:150
FACS 1:100
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.58 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human LIN7B (NP_071448) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lin-7 homolog B, crumbs cell polarity complex component , LIN-7B , MALS-2 , MALS2 , VELI2

Cellular Localization

Cell junction

Background

Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells. May increase the amplitude of ACCN3 acid-evoked currents by stabilizing the channel at the cell surface.

Database

Research Area

DATA IMAGES

Anti-LIN7B antibody [1C9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84216

GTX84216 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with LIN7B plasmid using GTX84216 LIN7B antibody [1C9].

Anti-LIN7B antibody [1C9] used in Flow cytometry (FACS). GTX84216

GTX84216 FACS Image

FACS analysis of HEK293T cells transfected with either LIN7B plasmid(Red) or empty vector control plasmid(Blue) using GTX84216 LIN7B antibody [1C9].

Anti-LIN7B antibody [1C9] used in IHC (Paraffin sections) (IHC-P). GTX84216

GTX84216 IHC-P Image

IHC-P analysis of human bladder carcinoma tissue using GTX84216 LIN7B antibody [1C9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-LIN7B antibody [1C9] used in Western Blot (WB). GTX84216

GTX84216 WB Image

WB analysis of HEK293T cells transfected with LIN7B plasmid (Right) or empty vector (Left) for 48 hrs using GTX84216 LIN7B antibody [1C9].
Loading : 5 ug per lane

Anti-LIN7B antibody [1C9] used in IHC (Paraffin sections) (IHC-P). GTX84216

GTX84216 IHC-P Image

IHC-P analysis of human lymph node tissue using GTX84216 LIN7B antibody [1C9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-LIN7B antibody [1C9] used in IHC (Paraffin sections) (IHC-P). GTX84216

GTX84216 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84216 LIN7B antibody [1C9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519